Teprenone, but not H2-receptor blocker or sucralfate, suppresses corpus Helicobacter pylori colonization and gastritis in humans: teprenone inhibition of H. pylori-induced interleukin-8 in MKN28 gastric epithelial cell lines
| Autor: | Katsuhiko Iwakiri, Tetsuro Hiratsuka, Seiji Futagami, Hitoshi Nishigaki, Ken Wada, Taku Tsukui, Kei Shinoki, Choitsu Sakamoto, Yoko Shinji, Nobutaka Yamada, Katya Gudis, Kazumasa Miyake |
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| Rok vydání: | 2004 |
| Předmět: |
Male
medicine.medical_specialty Biopsy Sucralfate Gastroenterology Cell Line Helicobacter Infections Internal medicine Pepsinogen A medicine Gastric mucosa Pepsinogen C Humans Helicobacter Interleukin 8 Nizatidine biology Helicobacter pylori business.industry Stomach Interleukin-8 Epithelial Cells General Medicine Middle Aged biology.organism_classification Anti-Ulcer Agents Urease Infectious Diseases medicine.anatomical_structure Histamine H2 Antagonists Neutrophil Infiltration Gastric Mucosa Gastritis Teprenone Female medicine.symptom Diterpenes business medicine.drug |
| Zdroj: | Helicobacter. 9(2) |
| ISSN: | 1083-4389 |
| Popis: | Background. The role of teprenone in Helicobacter pylori-associated gastritis has yet to be determined. To investigate the effect of teprenone on inflammatory cell infiltration, and on H. pylori colonization of the gastric mucosa in H. pylori-infected patients, we first compared the effect of teprenone with that of both histamine H2 receptor antagonists (H2-RA) and sucralfate on the histological scores of H. pylori gastritis. We then examined its in vitro effect on H. pylori-induced interleukin (IL)-8 production in MKN28 gastric epithelial cells. Materials and Methods. A total of 68 patients were divided into three groups, each group undergoing a 3-month treatment with either teprenone (150 mg/day), H2-RA (nizatidine, 300 mg/day), or sucralfate (3 g/day). All subjects underwent endoscopic examination of the stomach before and after treatment. IL-8 production in MKN28 gastric epithelial cells was measured by enzyme-linked immunosorbent assay (ELISA). Results. Following treatment, the teprenone group showed a significant decrease in both neutrophil infiltration and H. pylori density of the corpus (before vs. after: 2.49 ± 0.22 vs. 2.15 ± 0.23, p = .009; 2.36 ± 0.25 vs. 2.00 ± 0.24, p = .035, respectively), with no significant differences seen in either the sucralfate or H2-RA groups. Teprenone inhibited H. pylori-enhanced IL-8 production in MKN28 gastric epithelial cells in vitro, in a dose-dependent manner. Conclusions. Teprenone may modify corpus H. pylori-associated gastritis through its effect on neutrophil infiltration and H. pylori density, in part by its inhibition of IL-8 production in the gastric mucosa. |
| Databáze: | OpenAIRE |
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