Multistage bioassociation of uranium onto an extremely halophilic archaeon revealed by a unique combination of spectroscopic and microscopic techniques

Autor: Niculina Musat, Björn Drobot, Juliet S. Swanson, Andrea Cherkouk, Donald T. Reed, Matthias Schmidt, Katharina Müller, Harald Foerstendorf, Thorsten Stumpf, Miriam Bader
Rok vydání: 2017
Předmět:
Halobacterium
0301 basic medicine
Salinity
Environmental Engineering
Halophilic Archaea
Health
Toxicology and Mutagenesis
TRLFS
Halobacterium noricense
ved/biology.organism_classification_rank.species
Infrared spectroscopy
chemistry.chemical_element
010501 environmental sciences
Microscopy
Atomic Force
complex mixtures
01 natural sciences
03 medical and health sciences
Spectroscopy
Fourier Transform Infrared
Uranium Biosorption
Environmental Chemistry
Waste Management and Disposal
0105 earth and related environmental sciences
Microscopy
biology
ved/biology
Biosorption
Spectrometry
X-Ray Emission
Sorption
Hydrogen-Ion Concentration
Uranium
biology.organism_classification
Archaea
Pollution
Kinetics
Spectrometry
Fluorescence
X-Ray Absorption Spectroscopy
030104 developmental biology
chemistry
Ionic strength
Radioactive Waste
Attenuated total reflection
in situ ATR FT-IR
Microscopy
Electron
Scanning
Nuclear chemistry
Zdroj: Journal of Hazardous Materials 327(2017), 225-232
ISSN: 0304-3894
DOI: 10.1016/j.jhazmat.2016.12.053
Popis: The interactions of two extremely halophilic archaea with uranium were investigated at high ionic strength as a function of time, pH and uranium concentration. Halobacterium noricense DSM-15987 and Halobacterium sp. putatively noricense isolated from the Waste Isolation Pilot Plant (WIPP) repository were used for these investigations. The kinetics of U(VI) bioassociation of both strains showed an atypical multistage behavior, meaning that after an initial phase of U(VI) sorption, an unexpected interim period of U(VI) release was observed, followed by a slow reassociation of uranium with the cells. By applying in situ attenuated total reflection Fourier-transform infrared (ATR FT-IR) spectroscopy, the involvement of phosphoryl and carboxylate groups in U(VI) complexation during the first biosorption phase was shown. Differences in cell morphology and uranium localization become visible at different stages of the bioassociation process, by using scanning electron microscopy (SEM) in combination with energy dispersive X-ray (EDX) spectroscopy. Our results demonstrate for the first time that association of uranium on the extremely halophilic archaeon is a multistage process, beginning with a sorption which is followed by another process, probably biomineralization.
Databáze: OpenAIRE
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