A block to the intracellular transport and assembly of hepatitis B surface antigen polypeptides in Xenopus oocytes
| Autor: | Kay Simon, Don Ganem, Vishwanath R. Lingappa |
|---|---|
| Rok vydání: | 1988 |
| Předmět: |
HBsAg
Microinjections Xenopus Biology Endoplasmic Reticulum Xenopus laevis Virology medicine Animals Secretion Glycoproteins chemistry.chemical_classification Messenger RNA Hepatitis B Surface Antigens Membrane Glycoproteins Endoplasmic reticulum virus diseases Biological Transport Transfection biology.organism_classification Oocyte Molecular biology digestive system diseases Molecular Weight medicine.anatomical_structure chemistry Oocytes Glycoprotein Protein Processing Post-Translational |
| Zdroj: | Virology. 166(1) |
| ISSN: | 0042-6822 |
| Popis: | Hepatitis B surface antigen is the major protein of the virion envelope, and is also independently secreted from infected cells as a subviral particle composed exclusively of HBsAg and host-derived lipid. Similar particles are efficiently assembled and secreted by cultured mammalian cells transfected with the gene for HBsAg. In contrast to such cultured cells, Xenopus oocytes microinjected with HBsAg mRNA secrete less than 5% of newly synthesized HBsAg polypeptides. We have examined the HBsAg biosynthetic intermediates in such oocytes and provide evidence that the impaired secretion of HBsAg is due to a discrete block in the assembly of lipoprotein particles. |
| Databáze: | OpenAIRE |
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