Multiple copies of a novel amphipathic α-helix forming segment in Physcomitrella patens dehydrin play a key role in abiotic stress mitigation
| Autor: | Chandrima Chakraborty, Chandra Basak, Chandradeep Basu, Arup Das, Tanushree Agarwal, Sudipta Ray, Gouranga Upadhyaya, Gautam Basu, Tanmoy Halder |
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| Rok vydání: | 2021 |
| Předmět: |
0301 basic medicine
Protein Conformation alpha-Helical nuclear magnetic resonance (NMR) BiFC bimolecular fluorescence complementation IDP intrinsically disordered protein PpDHNA Physcomitrella patens dehydrin Protein aggregation cell survivability Physcomitrella patens Biochemistry NT nontransformed dehydrin–dehydrin interaction protein aggregation 03 medical and health sciences Bimolecular fluorescence complementation Stress Physiological Amphiphile VT vector transformed D-segment Molecular Biology amphipathic α-helix Plant Proteins TFE trifluoroethanol Abiotic component high-temperature stress MDA malondialdehyde 030102 biochemistry & molecular biology biology DHN dehydrin LDH lactate dehydrogenase Chemistry Abiotic stress LEA late embryogenesis abundant food and beverages Cell Biology biology.organism_classification intrinsically disordered protein Bryopsida Cell biology 030104 developmental biology Helix Desiccation poikilohydric plant Research Article self-association |
| Zdroj: | The Journal of Biological Chemistry |
| ISSN: | 1083-351X |
| Popis: | Plants use a diverse set of proteins to mitigate various abiotic stresses. The intrinsically disordered protein dehydrin is an important member of this repertoire of proteins, characterized by a canonical amphipathic K-segment. It can also contain other stress-mitigating noncanonical segments—a likely reflection of the extremely diverse nature of abiotic stress encountered by plants. Among plants, the poikilohydric mosses have no inbuilt mechanism to prevent desiccation and therefore are likely to contain unique noncanonical stress-responsive motifs in their dehydrins. Here we report the recurring occurrence of a novel amphipathic helix-forming segment (D-segment: EGφφD(R/K)AKDAφ, where φ represents a hydrophobic residue) in Physcomitrella patens dehydrin (PpDHNA), a poikilohydric moss. NMR and CD spectroscopic experiments demonstrated the helix-forming tendency of the D-segment, with the shuffled D-segment as control. PpDHNA activity was shown to be size as well as D-segment dependent from in vitro, in vivo, and in planta studies using PpDHNA and various deletion mutants. Bimolecular fluorescence complementation studies showed that D-segment-mediated PpDHNA self-association is a requirement for stress abatement. The D-segment was also found to occur in two rehydrin proteins from Syntrichia ruralis, another poikilohydric plant like P. patens. Multiple occurrences of the D-segment in poikilohydric plant dehydrins/rehydrins, along with the experimental demonstration of the role of D-segment in stress abatement, implies that the D-segment mediates unique resurrection strategies, which may be employed by plant dehydrins that are capable of mitigating extreme stress. |
| Databáze: | OpenAIRE |
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