Transforming growth factor β as a neuronoglial signal during peripheral nervous system response to injury
| Autor: | B. Rogister, P. Delrée, P. Leprince, D. Martin, C. Sadzot, B. Malgrange, C. Munaut, J. M. Rigo, P. P. Lefebvre, J.-N. Octave, J. Schoenen, G. Moonen |
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| Rok vydání: | 1993 |
| Předmět: |
Schwann cell
Biology Schwann cell proliferation Plasminogen Activators Cellular and Molecular Neuroscience Peripheral Nerve Injuries Transforming Growth Factor beta Ganglia Spinal TGF beta signaling pathway medicine Animals Nerve Growth Factors Peripheral Nerves RNA Messenger Cells Cultured Neurons Extracellular Matrix Proteins Transforming growth factor beta Endoglin Immunohistochemistry Culture Media Rats Cell biology Plasminogen Inactivators medicine.anatomical_structure Nerve growth factor nervous system Transforming growth factor beta 3 biology.protein Neuroglia Schwann Cells Neuroscience Cell Division Signal Transduction |
| Zdroj: | Journal of Neuroscience Research. 34:32-43 |
| ISSN: | 1097-4547 0360-4012 |
| DOI: | 10.1002/jnr.490340105 |
| Popis: | In contrast to the central nervous system (CNS), the peripheral nervous system (PNS) displays an important regenerative ability which is dependent, at least in part, on Schwann cell properties. The mechanisms which stimulate Schwann cells to adapt their behavior after a lesion to generate adequate conditions for PNS regeneration remain unknown. In this work, we report that adult rat dorsal root ganglion (DRG) neurons are able, after a lesion performed in vivo or when they are dissociated and cultured in vitro, to synthesize transforming growth factor beta (TGF beta), a pleiotropic growth factor implicated in wound healing processes and in carcinogenesis. This TGF beta is tentatively identified as the beta-1 isoform. Adult rat DRG neurons release a biologically active form of TGF beta which is able to elicit multiple Schwann cell responses including a stimulation to proliferate. Moreover, purified TGF beta-1 produces a Schwann cell morphology alteration and decreases the secretion of tissue-type plasminogen activator (tPA) and enhances the secretion of plasminogen activator inhibitor (PAI) by Schwann cells. This generates conditions which are thought to favor a successful neuritic regrowth. Furthermore, purified TGF beta-1 stimulates type IV collagen mRNA expression in Schwann cells. This subtype of collagen is associated with the process of myelinization. Finally, TGF beta-1 decreases nerve growth factor (NGF) mRNA expression by Schwann cells, an effect which could participate in the maintenance of a distoproximal NGF gradient during nerve regeneration. We propose that neuronal TGF beta plays an essential role as a neuronoglial signal that modulates the response of Schwann cells to injury and participates in the successful regeneration processes observed in the PNS. |
| Databáze: | OpenAIRE |
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