Increased E2F1 mRNA and miR-17-5p Expression Is Correlated to Invasiveness and Proliferation of Pituitary Neuroendocrine Tumours
| Autor: | Antonio Pico, Araceli García-Martínez, Carmen Fajardo, Sandra Silva-Ortega, Rosa Cámara, Beatriz López-Muñoz, Ignacio Aranda, Cristina Lamas |
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| Rok vydání: | 2020 |
| Předmět: |
0301 basic medicine
endocrine system Somatotropic cell E2F1 Clinical Biochemistry Article 03 medical and health sciences 0302 clinical medicine microRNA Medicine pituitary neuroendocrine tumours Messenger RNA lcsh:R5-920 business.industry Mir 17 5p Cancer medicine.disease microRNAs 030104 developmental biology 030220 oncology & carcinogenesis Cancer research Biomarker (medicine) miR-17~92 Corticotropic cell business lcsh:Medicine (General) |
| Zdroj: | DIAGNOSTICS r-FISABIO: Repositorio Institucional de Producción Científica Fundación para el Fomento de la Investigación Sanitaria y Biomédica de la Comunitat Valenciana (FISABIO) r-IIS La Fe. Repositorio Institucional de Producción Científica del Instituto de Investigación Sanitaria La Fe instname Diagnostics r-FISABIO. Repositorio Institucional de Producción Científica Diagnostics, Vol 10, Iss 227, p 227 (2020) Volume 10 Issue 4 r-ISABIAL. Repositorio Institucional de Producción Científica del Instituto de Investigación Biomédica y Sanitaria de Alicante |
| ISSN: | 2075-4418 |
| Popis: | miR-17-5p and E2F1 have been described as deregulated in cancer, but they have scarcely been studied in pituitary neuroendocrine tumours (PitNETs). This study evaluates the relationship of E2F1 and miR-17-5p with the invasiveness and proliferation of PitNETs. In this cross-sectional descriptive study, we evaluated the expression of E2F1, MYC, and miR-17-5p by quantitative real time PCR analysis in 60 PitNETs: 29 gonadotroph (GT), 15 functioning somatotroph (ST), and 16 corticotroph (CT) tumours, of which 8 were silent (sCT). The clinical data were collected from the Spanish Molecular Register of Pituitary Adenomas (REMAH) database. We defined invasiveness according to the Knosp classification and proliferation according to a molecular expression of Ki-67 &ge 2.59. E2F1 was more expressed in invasive than in non-invasive tumours in the whole series (p = 0.004) and in STs (p = 0.01). In addition, it was overexpressed in the silent subtypes (GTs and sCTs all macroadenomas) and normoexpressed in the functioning ones (fCTs and STs some microadenomas). miR-17-5p was more expressed in proliferative than in non-proliferative tumours (p = 0.041) in the whole series but not by subtypes. Conclusions: Our study suggests that in PitNETs, E2F1 could be a good biomarker of invasiveness, and miR-17-5p of proliferation, helping the clinical management of these tumours. |
| Databáze: | OpenAIRE |
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