Day-Time Isoflurane Administration Suppresses Circadian Gene Expressions in Both the Brain and a Peripheral Organ, Liver
| Autor: | Necati Gokmen, Ali Günerli, Ibrahim Halil Kavakli, Osman Yilmaz, Elvan Öçmen, Ibrahim Baris |
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| Přispěvatelé: | Barış, ibrahim (ORCID 0000-0003-2185-3259 & YÖK ID 111629), Kavaklı, İbrahim H. (ORCID 0000-0001-6624-3505 & YÖK ID 40319), Gökmen, Necati, Barış, İbrahim, Öçmen, Elvan, Yılmaz, Osman, Günerli, Ali, School of Medicine, College of Engineering, Department of Molecular Biology, Department of Chemical and Biological Engineering |
| Rok vydání: | 2017 |
| Předmět: |
0301 basic medicine
Medicine Anesthesiology medicine.medical_specialty business.industry Circadian clock Reverse transcription polymerase chain reaction PER2 CLOCK 03 medical and health sciences 030104 developmental biology 0302 clinical medicine Endocrinology Anaesthesia Circadian rhythm Clock genes Isoflurane Internal medicine Gene expression Zeitgeber medicine Original Article business 030217 neurology & neurosurgery medicine.drug |
| Zdroj: | Turk Anesteziyoloji Ve Reanimasyon Dernegi Dergisi |
| ISSN: | 2149-276X 2149-0937 |
| DOI: | 10.5152/tjar.2017.68466 |
| Popis: | Objective: The aim of this study is to investigate the effects of light and administration time of isoflurane on circadian gene expression in the brains and liver tissues of rats kept in light-dark cycle. Methods: Seventy tw o 15-days-old rats pups were divided into four groups. All animals were exposed to 1.5% concentration of isoflurane or to 6 L min(-1) O-2 for six hours between Zeitgeber Time (ZT) 0-ZT06 ( day-time administration) or ZT12-ZT18 ( night-time administration). Rats were sacrificed after six hours of anaesthesia with four-hour time intervals. Total RNA was isolated from brains and liver tissues. Circadian gene expression was examined using quantitative real-time Reverse transcription polymerase chain reaction (RT-PCR). Results: BMAL1, CLOCK, PER2 and CRY2 gene expression levels were markedly suppressed after day-time anaesthesia in the both brain and liver, but night-time administration caused only temporary suppression of gene expression. Conclusion: The effect of isoflurane on the circadian clock is time-dependent, and administered isoflurane anaesthesia at night had minimal effect on clock gene expression. Additionally, when the treated animals were kept in a regular light-dark cycle, isoflurane-induced phase shift was not observed, possibly because of the light. NA |
| Databáze: | OpenAIRE |
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