Circular RNA FLNA acts as a sponge of miR-486-3p in promoting lung cancer progression via regulating XRCC1 and CYP1A1
| Autor: | Jiongwei Pan, Enhui Gong, Zhuo Cao, Zhangyong Yin, Wei Cheng, Zaiting Ye, Yuling Li, Gang Huang, Xiaoping Cai, Cunlai Xu |
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| Rok vydání: | 2021 |
| Předmět: |
Cancer Research
Lung Neoplasms Filamins Biology Article Metastasis Mice Western blot Cell Line Tumor Genetics Cytochrome P-450 CYP1A1 medicine Animals Humans Lung cancer Molecular Biology Cancer Cell Proliferation Gene knockdown medicine.diagnostic_test RNA Circular Transfection respiratory system medicine.disease Gene Expression Regulation Neoplastic MicroRNAs X-ray Repair Cross Complementing Protein 1 Apoptosis Cancer cell Cancer research Molecular Medicine Immunohistochemistry |
| Zdroj: | Cancer Gene Therapy |
| ISSN: | 1476-5500 0929-1903 |
| DOI: | 10.1038/s41417-021-00293-w |
| Popis: | Significantly high-expressed circFLNA has been found in various cancer cell lines, but not in lung cancer. Therefore, this study aimed to explore the role of circFLNA in the progression of lung cancer. The target gene of circFLNA was determined by bioinformatics and luciferase reporter assay. Viability, proliferation, migration, and invasion of the transfected cells were detected by CCK-8, colony formation, wound-healing, and transwell assays, respectively. A mouse subcutaneous xenotransplanted tumor model was established, and the expressions of circFLNA, miR-486-3p, XRCC1, CYP1A1, and related genes in the cancer cells and tissues were detected by RT-qPCR, Western blot, or immunohistochemistry. The current study found that miR-486-3p was low-expressed in lung cancer. MiR-486-3p, which has been found to target XRCC1 and CYP1A1, was regulated by circFLNA. CircFLNA was located in the cytoplasm and had a high expression in lung cancer cells. Cancer cell viability, proliferation, migration, and invasion were promoted by overexpressed circFLNA, XRCC1, and CYP1A1 but inhibited by miR-486-3p mimic and circFLNA knockdown. The weight of the xenotransplanted tumor was increased by circFLNA overexpression yet reduced by miR-486-3p mimic. Furthermore, miR-486-3p mimic reversed the effect of circFLNA overexpression on promoting lung cancer cells and tumors and regulating the expressions of miR-486-3p, XRCC1, CYP1A1, and metastasis/apoptosis/proliferation-related factors. However, overexpressed XRCC1 and CYP1A1 reversed the inhibitory effect of miR-486-3p mimic on cancer cells and tumors. In conclusion, circFLNA acted as a sponge of miR-486-3p to promote the proliferation, migration, and invasion of lung cancer cells in vitro and in vivo by regulating XRCC1 and CYP1A1. |
| Databáze: | OpenAIRE |
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