Avian leukosis virus subgroup J induces its receptor--chNHE1 up-regulation
| Autor: | Wei Meng, Weiguo Feng, Xiyao Cui, Ziqiang Cheng, Guihua Wang, Zhifang Pan, Liming Cai |
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| Rok vydání: | 2016 |
| Předmět: |
0301 basic medicine
Sodium-Hydrogen Exchangers Avian leukosis virus subgroup J (ALV-J) Chicken sodium hydrogen exchanger isoform 1 (chNHE1) Viremia Biology Real-Time Polymerase Chain Reaction Virus Immune tolerance 03 medical and health sciences 0302 clinical medicine Retrovirus Cell surface receptor Virology medicine Animals Viral shedding Receptor Avian Leukosis Virus Gene Expression Profiling Research Hydrogen-Ion Concentration biology.organism_classification medicine.disease Immunohistochemistry Up-Regulation 030104 developmental biology Real-time polymerase chain reaction Infectious Diseases Spectrometry Fluorescence 030220 oncology & carcinogenesis Host-Pathogen Interactions Receptors Virus Chickens |
| Zdroj: | Virology Journal |
| ISSN: | 1743-422X |
| Popis: | s Background Avian leukosis virus subgroup J (ALV-J) is an oncogenic retrovirus which causes immunosuppression and neoplasia in meat-type and egg-type chickens. ALV-J infects host cells via specific interaction between the viral Env and the cell surface receptor —chicken sodium hydrogen exchanger type 1 (chNHE1). NHE1 involved in altering the cellular pH and playing a critical role in tumorigenesis. However, little is known about the other relationship between ALV-J and chNHE1. Methods and results In ALV-J infected DF-1 cells, the mRNA level of chNHE1 was up-regulated with time-dependent manner tested by real time PCR, and accordingly, intracellular pH was increased tested by spectrofluorometer. In vivo, the mRNA level of chNHE1 was determined by real time PCR in ALV-J infected experimental chickens and field cases. The result showed that the mRNA level of chNHE1 was up-regulated after virus shedding, especially in continuous viremic shedders (CS group). However, no significant difference was found between non-shedding group (NS group) and control group. In field cases, mRNA level of chNHE1 was positively correlated with increasing ALV-J load in tumor bearing and immune tolerance chickens. Furthermore, immunohistochemistry results showed that the protein expression of chNHE1 was up-regulated in different organs of both experimental chickens and tumor bearing chickens compared with the control. Conclusion Taken together, we conclude that ALV-J induces chNHE1 up-regulation in viremia and neoplasia chickens. |
| Databáze: | OpenAIRE |
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