Evidence for the involvement of oxygen free radicals in the ethanol-induced late cellular injury in mouse myeloma cells
| Autor: | Beáta Bódis, Péter Németh, Gyula Mózsik, Imre Szabó, O. Karádi, József Belágyi |
|---|---|
| Rok vydání: | 2000 |
| Předmět: |
Time Factors
Ethanol Dose-Response Relationship Drug Spin trapping Dimethyl sulfoxide General Neuroscience Radical Osmolar Concentration Electron Spin Resonance Spectroscopy Free Radical Scavengers Glutathione Molecular biology Mice chemistry.chemical_compound chemistry Biochemistry Cell culture Physiology (medical) Tumor Cells Cultured Animals Viability assay Multiple Myeloma Reactive Oxygen Species Incubation |
| Zdroj: | Journal of Physiology-Paris. 94:67-70 |
| ISSN: | 0928-4257 |
| Popis: | In this study, we analysed the ethanol-induced long term cell injury on a general cell model (Sp2/0-Ag14 cell line). Cells were incubated in 1, 5, 10, 15 and 20% of ethanol (EtOH) for 5 min. After washing cell viability was tested by the Trypan Blue exclusion test in 5, 60 min, 4 and 24 h after EtOH exposure. Free radicals were monitored every 30 min by electron spin resonance (ESR) with alpha-phenyl-N-tert-butylnitrone (PBN) spin trapping technique. Scavenger compounds such as glutathione (GSH), dimethyl sulfoxide (DMSO) and 5,5-dimethyl-1-pyrroline N-oxide (DMPO) were applied for 24 h incubation after EtOH exposure. EtOH concentration dependently decreased the cell viability immediately after 5 min exposure, but with 4 and 24 h, a secondary cell destruction was found. Using ESR-spin trapping technique, an increased free radical activity could be detected. DMPO, DMSO and GSH significantly, but in different period protected the cells against free-radical induced cellular damage. EtOH produces an early (immediately after EtOH exposure) and a late (in about 4 h) cellular damage on Sp2/0-Ag14 cells. The oxygen free radicals can be detected in a short time after EtOH exposure, its biological effect manifested as a secondary cell destruction at 4 and 24 h. This phenomenon can be prevented by scavenger compounds. |
| Databáze: | OpenAIRE |
| Externí odkaz: |
|
Full Text from ScienceDirect