m-Octopamine: Normal Occurrence with p-Octopamine in Mammalian Sympathetic Nerves
| Autor: | Melvin J. Fregly, C. M. Williams, M. W. Couch, K. E. Ibrahim, John M. Midgley |
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| Rok vydání: | 1985 |
| Předmět: |
Male
medicine.medical_specialty Monoamine Oxidase Inhibitors Sympathetic Nervous System Monoamine oxidase Biochemistry Gas Chromatography-Mass Spectrometry Cellular and Molecular Neuroscience chemistry.chemical_compound In vivo Internal medicine Biogenic amine Phenethylamines medicine Animals Neurotransmitter Octopamine chemistry.chemical_classification 2-Hydroxyphenethylamine Kidney Synephrine Chemistry Vas deferens Rats Inbred Strains Rats medicine.anatomical_structure Endocrinology Octopamine (neurotransmitter) medicine.drug |
| Zdroj: | Journal of Neurochemistry. 44:1862-1867 |
| ISSN: | 1471-4159 0022-3042 |
| DOI: | 10.1111/j.1471-4159.1985.tb07180.x |
| Popis: | The development of a radiochemical enzyme assay for p-octopamine in 1969 led to its identification in a large number of invertebrate nerve systems and in mammalian sympathetic nerves. The original method by which p-octopamine was measured has now been found to be nonspecific; however, modifications of this procedure can determine both m- and p-octopamine. We recently developed a new specific method for the unequivocal identification and quantitative determination in tissue of the six octopamine and synephrine isomers. With this method—negative chemical ionization gas chromatography-mass spectrometry—the more physiologically active m-octopamine has been found in association with p-octopamine in 10 organs of the rat. m-Octopamine is present in concentrations equal to those of p-octopamine in heart, spleen, and liver and in concentrations from 30 to 60% of p-octopamine in adrenals, vas deferens, brain, kidney, large intestine, bladder, and lungs. In vivo inhibition of monoamine oxidase markedly increased the concentrations of both m- and p-octopamine in all organs examined. Both amines were virtually absent from all organs except the adrenals following chemical sympathectomy with 6-hydroxydopamine, thereby establishing that m- and p-octopamine are localized within sympathetic nerve endings. |
| Databáze: | OpenAIRE |
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