The scid mutation in mice causes a general defect in DNA repair
| Autor: | Robert A. Phillips, G. M. Fulop |
|---|---|
| Rok vydání: | 1990 |
| Předmět: |
DNA Repair
Cell Survival DNA repair DNA damage Lymphocyte In Vitro Techniques Biology Gene Rearrangement T-Lymphocyte medicine.disease_cause Colony-Forming Units Assay Mice Bone Marrow medicine Animals Gene Rearrangement B-Lymphocyte Receptor Mutation Micronucleus Tests Multidisciplinary T-cell receptor Immunologic Deficiency Syndromes T lymphocyte Gene rearrangement Molecular biology Mice Mutant Strains medicine.anatomical_structure Gamma Rays DNA Damage |
| Zdroj: | Nature. 347:479-482 |
| ISSN: | 1476-4687 0028-0836 |
| DOI: | 10.1038/347479a0 |
| Popis: | Mice homozygous for the scid mutation on chromosome 16 have a severe combined immune deficiency as a result of their inability to correctly rearrange their immunoglobulin and T-cell receptor genes. In scid mice, when precursors for B and T lymphocytes reach the stage of development requiring expression of these surface receptors, a defective recombinase system aberrantly cuts and rejoins the receptor gene segments greatly reducing the efficiency of producing functional receptors. As a result, most scid mice have no detectable B or T lymphocytes. We have demonstrated that the scid defect is not specific to lymphocyte development. Myeloid cells and fibroblasts from scid mice show a marked increase in sensitivity to ionizing radiation, indicating that the scid mutation leads to an inability to repair DNA damage induced by ionizing radiation as well as interfering with rearrangement of the immunoglobulin and T-cell receptor genes. |
| Databáze: | OpenAIRE |
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