Chromosome clustering by Ki-67 excludes cytoplasm during nuclear assembly
| Autor: | Alberto Hernandez-Armendariz, Maximilian W. G. Schneider, Sara Cuylen-Haering, Daniel W. Gerlich, Mina Petrovic, Matthias Samwer, Liam J. Holt, Claudia Blaukopf |
|---|---|
| Jazyk: | angličtina |
| Rok vydání: | 2020 |
| Předmět: |
Cytoplasm
Nuclear Envelope Physical Distancing Mitosis Spindle Apparatus Article Chromosomes 03 medical and health sciences 0302 clinical medicine medicine Chromosomes Human Humans Cluster Analysis Chromosome Positioning 030304 developmental biology Anaphase 0303 health sciences Multidisciplinary Chemistry Biological Transport Spindle apparatus Cell biology Cytosol medicine.anatomical_structure Ki-67 Antigen Mitotic exit Nucleus Ribosomes 030217 neurology & neurosurgery HeLa Cells |
| Zdroj: | Nature |
| ISSN: | 1476-4687 0028-0836 |
| Popis: | Gene expression in eukaryotes requires the effective separation of nuclear transcription and RNA processing from cytosolic translation1. This separation is achieved by the nuclear envelope, which controls the exchange of macromolecules through nuclear pores2. During mitosis, however, the nuclear envelope in animal and plant cells disassembles, allowing cytoplasmic and nuclear components to intermix3. When the nuclear envelope is reformed, cytoplasmic components are removed from the nucleus by receptor-mediated transport through nuclear pores2. These pores have a size limit of 39 nanometres4–7, which raises the question of how larger cytoplasmic molecules are cleared from the nucleus. Here we show in HeLa cells that large cytoplasmic components are displaced before nuclear envelope assembly by the movement of chromosomes to a dense cluster. This clustering occurs when chromosomes approach the poles of anaphase spindles, and is mediated by a microtubule-independent mechanism that involves the surfactant-like protein Ki-67. Ki-67 forms repulsive molecular brushes during the early stages of mitosis8, but during mitotic exit the brushes collapse and Ki-67 promotes chromosome clustering. We show that the exclusion of mature ribosomes from the nucleus after mitosis depends on Ki-67-regulated chromosome clustering. Thus, our study reveals that chromosome mechanics help to re-establish the compartmentalization of eukaryotic cells after open mitosis. The surfactant-like protein Ki-67 mediates the clustering of chromosomes during mitotic exit, which displaces large cytoplasmic molecules from the future nuclear space and thus enables the separation of cytoplasmic and nuclear components before the nuclear envelope reforms. |
| Databáze: | OpenAIRE |
| Externí odkaz: |
|
Full text from SpringerLink