Expression of Periostin in Cancer-associated Fibroblasts in Mammary Cancer in Female Dogs
Autor: | Aleksandra Piotrowska, Piotr Dziegiel, Katarzyna Ratajczak-Wielgomas, Małgorzata Kandefer-Gola, Marcin Nowak, Rafał Ciaputa, Izabela Janus, Marzena Podhorska-Okolow, Alicja M. Kmiecik, P. Borecka |
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Jazyk: | angličtina |
Rok vydání: | 2020 |
Předmět: |
Cancer Research
Adenoma Gene Expression Vimentin Mammary Neoplasms Animal Periostin General Biochemistry Genetics and Molecular Biology Metastasis 03 medical and health sciences 0302 clinical medicine Dogs Stroma Cancer-Associated Fibroblasts medicine Carcinoma Animals Pharmacology biology business.industry Cancer medicine.disease Immunohistochemistry 030220 oncology & carcinogenesis Cancer research biology.protein Female Neoplasm Grading business Cell Adhesion Molecules Biomarkers Research Article |
Zdroj: | In Vivo |
Popis: | Background/aim Mammary neoplasms, like breast neoplasms in women, are one of the most common tumours in female dogs. Cancer-associated fibroblasts (CAFs) found in the tumour stroma play a role in angiogenesis and increase cell migration, contributing to tumour growth and progression, as well as metastasis. The aim of our work was to determine the level of periostin (POSTN) expression in CAFs in mammary tumours of female dogs. Materials and methods The research material consisted of 77 carcinomas and 24 adenomas of the mammary ridge in female dogs. Immunohistochemistry tests were performed using antibodies directed against the antigens POSTN, Ki-67, ERB-B2 receptor tyrosine kinase 2 (HER2), vimentin, and alpha smooth muscle actin (αSMA). Expression of POSTN at the mRNA level was determined using real-time polymerase chain reaction methods in 20 cases of mammary neoplasms. Results Expression of POSTN in CAFs was observed in 92% of mammary cancer samples and in 25% of mammary adenoma samples in female dogs. A statistically significant increase in POSNT expression in CAFs was found in the carcinomas compared with mammary adenomas in female dogs. Expression of POSTN in CAFs in mammary carcinomas in female dogs positively correlated with the histological malignancy grade of tumours and the expression of Ki-67 proliferative antigen. Conclusion Our results suggest a role of POSTN on the pathogenesis of mammary tumours in female dogs. Moreover, POSTN may prove to be a useful marker in the evaluation of cancerous stroma of mammary tumours in female dogs, and may have prognostic significance. |
Databáze: | OpenAIRE |
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