Improvement of loperamide-induced slow transit constipation by Bifidobacterium bifidum G9-1 is mediated by the correction of butyrate production and neurotransmitter profile due to improvement in dysbiosis

Autor: Miyuki Yamamoto, Yutaka Makizaki, Taiki Uemoto, Haruka Yokota, Hiroshi Ohno, Yoshiki Tanaka
Jazyk: angličtina
Rok vydání: 2021
Předmět:
Constipation
Dopamine
ved/biology.organism_classification_rank.species
Pharmacology
Gut flora
Tryptophan Hydroxylase
Pathology and Laboratory Medicine
Biochemistry
law.invention
Probiotic
Feces
law
Medicine and Health Sciences
Neurotransmitter Agents
Multidisciplinary
biology
Gastrointestinal Motility Disorders
Neurochemistry
Neurotransmitters
Butyrates
Laxatives
Medicine
medicine.symptom
Anatomy
Pathogens
medicine.drug
Research Article
Pathogen Motility
Loperamide
Serotonin
Colon
Virulence Factors
Science
Butyrate
Gastroenterology and Hepatology
Microbiology
Gene Expression Regulation
Enzymologic
Signs and Symptoms
medicine
Animals
Humans
Bifidobacterium bifidum
Bacteria
ved/biology
business.industry
Probiotics
Gut Bacteria
Organisms
Biology and Life Sciences
biology.organism_classification
medicine.disease
Gastrointestinal Microbiome
Rats
Gastrointestinal Tract
Disease Models
Animal
Quality of Life
Butyric Acid
Dysbiosis
Clinical Medicine
business
Gastrointestinal Motility
Digestive System
Neuroscience
Zdroj: PLoS ONE, Vol 16, Iss 3, p e0248584 (2021)
PLoS ONE
ISSN: 1932-6203
Popis: A treatment option for constipation that improves the quality of life is needed since available laxatives do not effectively improve the quality of life in patients with constipation. A significant association between gut dysbiosis and constipation is recognized, suggesting that probiotics may be an important option for management of constipation. The underlying mechanism by which probiotics improve constipation remains unclear. In this study, we aimed to evaluate the effects of the probioticBifidobacterium bifidumG9-1 (BBG9-1) on loperamide-induced delayed colonic transit constipation and to elucidate its mechanism of action. First, the effect of BBG9-1 was evaluated in a rat model of constipation induced by subcutaneous administration of loperamide. BBG9-1 improved constipation parameters (number of feces, fecal water content, and fecal hardness) in constipated rats. Next, the relationship of organic acids and neurotransmitters to gut microbiota was investigated. BBG9-1 improved dysbiosis and prevented a decrease in butyric acid concentration in the gut, increased serum serotonin, and suppressed an increase in dopamine and a decrease in acetylcholine in serum. Further, an increase in the expression level of tryptophan hydroxylase 1, a 5-HT-synthetizing enzyme, was observed. These results suggest that BBG9-1 improves dysbiosis, which results in an increase in organic acids and improvement of neurotransmission. These actions may increase intestinal mobility, finally leading to alleviating constipation. The probiotic BBG9-1 may, therefore, be a potential option for the treatment of constipation.
Databáze: OpenAIRE
Externí odkaz: