Identification of Acyl-CoA Thioesterase in Mouse Mesenteric Lymph Nodes
| Autor: | Takayuki Ohtomo, Akemi Abe, Akio Mori, Niro Inaba, Osamu Kaminuma, Junji Yamada, Chisato Nakao, Tetsuta Kato, Megumi Sumiya |
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| Rok vydání: | 2013 |
| Předmět: |
Male
Pharmaceutical Science Adipose tissue Mice chemistry.chemical_compound Thioesterase medicine Animals Mesenteric lymph nodes Mesentery Pharmacology chemistry.chemical_classification Mice Inbred ICR Fatty acid metabolism biology Germinal center General Medicine Enzyme assay Enzyme medicine.anatomical_structure Palmitoyl-CoA Hydrolase chemistry Biochemistry ACOT7 biology.protein Lymph Nodes |
| Zdroj: | Biological and Pharmaceutical Bulletin. 36:866-871 |
| ISSN: | 1347-5215 0918-6158 |
| DOI: | 10.1248/bpb.b12-01088 |
| Popis: | Acyl-CoA thioesterases (ACOTs) are a group of enzymes that catalyze the hydrolysis of fatty acyl-CoAs to free fatty acids and CoA, with the potential to regulate the intracellular levels of these molecules. In this study, we show that a cytosolic isoform, ACOT7, is expressed at a significant level in the mesenteric lymph nodes (MLNs) of mice. While crude preparations of the mesenteric visceral fat contained significant levels of palmitoyl-CoA thioesterase activity, enzyme activity was concentrated 6.9-fold in MLNs compared with the residual adipose portion after excision of MLNs. When MLN homogenates were centrifuged, 82% of the enzyme activity was recovered in the cytosolic fraction, concomitant with almost exclusive recovery of ACOT7. Immunoprecipitation using anti-ACOT7 antibody estimated that 87% of enzyme activity in the homogenates was accounted for by ACOT7. On MLN sections, the germinal centers of secondary lymphoid follicles were immunostained with the antibody. In MLNs of mice fasted for 16 h, ACOT7 levels were induced 1.8-fold, which reflected a 1.5-fold increase in enzyme activity. These findings suggest that ACOT7 may be involved in dietary intake-associated responses in fatty acid metabolism in MLNs. |
| Databáze: | OpenAIRE |
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