Effect of Exogenous Melatonin on Hepatic Energetic Status during Ischemia/Reperfusion: Possible Role of Tumor Necrosis Factor-α and Nitric Oxide
| Autor: | Olivares N, Jesús Muñiz, Eliseo Portilla, Sergio Rodríguez-Reynoso, Caridad Leal |
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| Rok vydání: | 2001 |
| Předmět: |
Male
medicine.medical_specialty Cell Respiration Ischemia Nitric Oxide Synthase Type II Ketone Bodies Nitric Oxide Antioxidants Gene Expression Regulation Enzymologic Acetoacetates Nitric oxide Rats Sprague-Dawley Melatonin Lipid peroxidation chemistry.chemical_compound Internal medicine medicine Animals Aspartate Aminotransferases RNA Messenger Energy charge Nitrites 3-Hydroxybutyric Acid biology Tumor Necrosis Factor-alpha Alanine Transaminase medicine.disease Mitochondria Rats Survival Rate Nitric oxide synthase Endocrinology Liver chemistry Reperfusion Injury Ketone bodies biology.protein Surgery Tumor necrosis factor alpha Nitric Oxide Synthase Energy Metabolism medicine.drug |
| Zdroj: | Journal of Surgical Research. 100:141-149 |
| ISSN: | 0022-4804 |
| Popis: | Background. This study was designed to determine if the very potent antioxidant melatonin is able to reduce organ damage and improve energetic status in an in situ liver ischemia/reperfusion model. Materials and methods. Total hepatic ischemia was induced in rats by occlusion of the hepatic artery, portal vein, and bile duct. A portojugular shunt was inserted. After 60 min of ischemia, reperfusion was established for a period of 120 min. Rats were assigned to one group receiving systemic melatonin administration or to another receiving only normal saline. Variables were observed at preischemia, after 60 min of ischemia, and at 30, 60, and 120 min of reperfusion. Results. Energy charge, measured as the arterial ketone body ratio, showed higher values in the melatonin group during the first 60 min of reperfusion. Rises in plasma nitrite, tumor necrosis factor (TNF)-α, aspartate aminotransferase, alanine aminotransferase, lipid peroxidation products, and inducible nitric oxide synthase (iNOS) expression were less severe with melatonin. Linear regression analysis demonstrated a significant correlation between nitrites and arterial ketone body ratio (R2 = 0.2454). Bile production was higher with melatonin. Seven-day survival rates were 52% for control, 80% for melatonin, and 100% for sham groups. Conclusions. Exogenous melatonin is capable of preserving the functional and energetic status during ischemia/reperfusion which is associated with reduced concentrations of TNF-α and inhibited expression of iNOS and NO production. This improvement may be due to an adequate preservation of the hepatic mitochondrial redox status. |
| Databáze: | OpenAIRE |
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