Oxidative Stress, DNA Damage and DNA Repair in Female Patients with Diabetes Mellitus Type 2
| Autor: | Helmut Brath, Bianca Guggenberger, Annemarie Grindel, Anela Tosevska, David Briskey, Christina Pöppelmeyer, Lukas Eichberger, Michaela Gschaider, Karl-Heinz Wagner, George Mare |
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| Rok vydání: | 2016 |
| Předmět: |
0301 basic medicine
Physiology lcsh:Medicine Blood Pressure Xenopus Proteins medicine.disease_cause Biochemistry Antioxidants Body Mass Index DNA Ligase ATP chemistry.chemical_compound XRCC1 Endocrinology Diabetes mellitus 0302 clinical medicine Malondialdehyde DNA-(Apurinic or Apyrimidinic Site) Lyase Diabetes diagnosis and management Poly-ADP-Ribose Binding Proteins lcsh:Science Aged 80 and over chemistry.chemical_classification F2-Isoprostanes Multidisciplinary Glutathione peroxidase Hematology Middle Aged Catalase Glutathione Body Fluids DNA-Binding Proteins Lipoproteins LDL Nucleic acids Blood 030220 oncology & carcinogenesis Female Anatomy Research Article Adult medicine.medical_specialty HbA1c DNA Ligases Endocrine Disorders DNA repair DNA damage Biology 03 medical and health sciences Internal medicine Genetics medicine Humans Hypoglycemic Agents Hemoglobin Triglycerides Aged Glycated Hemoglobin Medicine and health sciences Glutathione Peroxidase Biology and life sciences Superoxide Dismutase Cholesterol HDL lcsh:R Proteins Cholesterol LDL DNA Cell Biology APEX1 Diagnostic medicine Cross-Sectional Studies X-ray Repair Cross Complementing Protein 1 030104 developmental biology Diabetes Mellitus Type 2 Gene Expression Regulation chemistry Oxidative stress Metabolic Disorders Leukocytes Mononuclear lcsh:Q Gene expression Glycated hemoglobin |
| Zdroj: | PLoS ONE, Vol 11, Iss 9, p e0162082 (2016) PLoS ONE |
| ISSN: | 1932-6203 |
| Popis: | Background Diabetes mellitus type 2 (T2DM) is associated with oxidative stress which in turn can lead to DNA damage. The aim of the present study was to analyze oxidative stress, DNA damage and DNA repair in regard to hyperglycemic state and diabetes duration. Methods Female T2DM patients (n = 146) were enrolled in the MIKRODIAB study and allocated in two groups regarding their glycated hemoglobin (HbA1c) level (HbA1c≤7.5%, n = 74; HbA1c>7.5%, n = 72). In addition, tertiles according to diabetes duration (DD) were created (DDI = 6.94±3.1 y, n = 49; DDII = 13.35±1.1 y, n = 48; DDIII = 22.90±7.3 y, n = 49). Oxidative stress parameters, including ferric reducing ability potential, malondialdehyde, oxidized and reduced glutathione, reduced thiols, oxidized LDL and F2-Isoprostane as well as the activity of antioxidant enzymes superoxide dismutase, catalase and glutathione peroxidase were measured. Damage to DNA was analyzed in peripheral blood mononuclear cells and whole blood with single cell gel electrophoresis. DNA base excision repair capacity was tested with the modified comet repair assay. Additionally, mRNA expressions of nine genes related to base excision repair were analyzed in a subset of 46 matched individuals. Results No significant differences in oxidative stress parameters, antioxidant enzyme activities, damage to DNA and base excision repair capacity, neither between a HbA1c cut off />7.5%, nor between diabetes duration was found. A significant up-regulation in mRNA expression was found for APEX1, LIG3 and XRCC1 in patients with >7.5% HbA1c. Additionally, we observed higher total cholesterol, LDL-cholesterol, LDL/HDL-cholesterol, triglycerides, Framingham risk score, systolic blood pressure, BMI and lower HDL-cholesterol in the hyperglycemic group. Conclusion BMI, blood pressure and blood lipid status were worse in hyperglycemic individuals. However, no major disparities regarding oxidative stress, damage to DNA and DNA repair were present which might be due to good medical treatment with regular health checks in T2DM patients in Austria. |
| Databáze: | OpenAIRE |
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