Towards automated screening of two-dimensional crystals
| Autor: | Anchi Cheng, Joel Quispe, Albert Leung, Joseph S. Lam, Christian Suloway, Denis Fellmann, James Pulokas, Clinton S. Potter, Priyanka D. Abeyrathne, Bridget Carragher |
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| Rok vydání: | 2007 |
| Předmět: |
Crystallography
business.industry Computer science Electron crystallography Proteolipids Membrane Proteins Nanotechnology Robotics Automation Negative Staining Article Computational science Crystal Fully automated Microscopy Electron Transmission Structural Biology business Protein crystallization Crystallization Electron microscopic |
| Zdroj: | Journal of structural biology. 160(3) |
| ISSN: | 1095-8657 |
| Popis: | Screening trials to determine the presence of two-dimensional (2D) protein crystals suitable for three-dimensional structure determination using electron crystallography is a very labor-intensive process. Methods compatible with fully automated screening have been developed for the process of crystal production by dialysis and for producing negatively stained grids of the resulting trials. Further automation via robotic handling of the EM grids, and semi-automated transmission electron microscopic imaging and evaluation of the trial grids is also possible. We, and others, have developed working prototypes for several of these tools and tested and evaluated them in a simple screen of 24 crystallization conditions. While further development of these tools is certainly required for a turn-key system, the goal of fully automated screening appears to be within reach. |
| Databáze: | OpenAIRE |
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