The effect of age and non-steroidal anti-inflammatory drugs on human intestinal microbiota composition
| Autor: | Nina Rautonen, Lars Paulin, Harri Mäkivuokko, Kirsti Tiihonen, Soile Tynkkynen |
|---|---|
| Rok vydání: | 2009 |
| Předmět: |
Adult
Male Urologic Diseases Aging Guanine Firmicutes Digestive System Diseases Medicine (miscellaneous) DNA Ribosomal Cytosine Young Adult 03 medical and health sciences 0302 clinical medicine Lactobacillus Humans Young adult Collinsella Aged 030304 developmental biology Aged 80 and over 2. Zero hunger 0303 health sciences Nutrition and Dietetics biology Mental Disorders Ruminococcus Anti-Inflammatory Agents Non-Steroidal Bacteroidetes biology.organism_classification 3. Good health Intestines Butyrates Laxatives Ageing 030220 oncology & carcinogenesis Immunology Female Nervous System Diseases Roseburia |
| Zdroj: | British Journal of Nutrition. 103:227-234 |
| ISSN: | 1475-2662 0007-1145 |
| Popis: | Ageing has been suggested to cause changes in the intestinal microbial community. In the present study, the microbiota of a previously well-defined group of elderly subjects aged between 70 and 85 years, both non-steroidal anti-inflammatory drugs (NSAID) users (n9) and non-users (n9), were further compared with young adults (n14) with a mean age of 28 years, by two DNA-based techniques: percentage guanine+cytosine (%G+C) profiling and 16S rDNA sequencing. Remarkable changes in microbiota were described with both methods: compared with young adults a significant reduction in overall numbers of microbes in both elderly groups was measured. Moreover, the total number of microbes in elderly NSAID users was higher than in elderly without NSAID. In 16S rDNA sequencing, shifts in all major microbial phyla, such as lower numbers of Firmicutes and an increase in numbers of Bacteroidetes in the elderly were monitored. On the genus level an interesting link between reductions in the proportion of known butyrate producers belonging toClostridiumcluster XIVa, such asRoseburiaandRuminococcus, could be demonstrated in the elderly. Moreover, in the Actinobacteria group, lower numbers ofCollinsellaspp. were evident in the elderly subjects with NSAID compared both with young adults and the elderly without NSAID, suggesting that the use of NSAID along with age may also influence the composition of intestinal microbiota. Furthermore, relatively high numbers ofLactobacillusappeared only in the elderly subjects without NSAID. In general, the lowered numbers of microbial members in the major phyla, Firmicutes, together with changes in the epithelial layer functions can have a significant effect on the colon health of the elderly. |
| Databáze: | OpenAIRE |
| Externí odkaz: |
|