Testis-specific lactate dehydrogenase is expressed in somatic tissues of plateau pikas
| Autor: | Lian Wei, Deng-Bang Wei, Xiao-Jun Wang, Xin-Feng Rao, Duo-Wei Wang, Xin-Zhang Qi, Ben-Yuan Ma |
|---|---|
| Rok vydání: | 2012 |
| Předmět: |
Somatic cell
Ochotona curzoniae Qinghai-Tibet Plateau Biology Plateau (mathematics) NBT nitrobenzene thiocyanate chloride General Biochemistry Genetics and Molecular Biology Article chemistry.chemical_compound cDNA complementary DNA Plateau pika (Ochotona curzoniae) Lactate dehydrogenase medicine Pika Testis-specific lactate dehydrogenase C Hypoxia Somatic tissues PMS phenazine methosulfate IPTG isopropyl-β-d-thiogalactopyranoside Skeletal muscle Anatomy CRE cAMP-response element biology.organism_classification Molecular biology Sperm LDH-C4 testis-specific lactate dehydrogenase medicine.anatomical_structure chemistry EST expressed sequence tag Immunohistochemistry |
| Zdroj: | FEBS Open Bio |
| ISSN: | 2211-5463 |
| Popis: | LDH-C4 is a lactate dehydrogenase that catalyzes the interconversion of pyruvate with lactate. In mammals the, Ldh-c gene was originally thought to be expressed only in testis and spermatozoa. Plateau pika (Ochotona curzoniae), belonging to the genus Ochotona of the Ochotonidea family, is a hypoxia tolerant mammal living at 3000–5000 m above sea levelon the Qinghai-Tibet Plateau. We found that the expression pattern of six LDH isoenzymes in the somatic tissues of female and male plateau pikas to be the same as those in testis and sperm, suggesting that LDH-C4 was expressed in somatic tissues of plateau pika. Here we report the detection of LDHC in the somatic tissues of plateau pika using RT-PCR, Western blotting and immunohistochemistry. Our results indicate that Ldh-c mRNA is transcribed in the heart, liver, lung, kidney, brain, skeletal muscle and testis. In somatic tissues LDHC was translated in the cytoplasm, while in testis it was expressed in both cytoplasm and mitochondria. The third band from cathode to anode in LDH isoenzymes was identified as LDH-C4. The finding that Ldh-c is expressed in both somatic tissues and testis of plateau pika provides important implications for more in-depth research into the Ldh-c function in mammals. Highlights ▸ Plateau pika (Ochotona curzoniae) express 6 isoforms of lactate dehydrogenase (LDH). ▸ LDH-C4 was thought to be expressed only in the testis and sperm of mammals. ▸ We detected LDH-C4 in somatic tissues as well as the testis and sperm of plateau pika. |
| Databáze: | OpenAIRE |
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