Sexually Transmitted Infections Detected by Multiplex Real Time PCR in Asymptomatic Women and Association with Cervical Intraepithelial Neoplasia
| Autor: | Carolina René Hoelzle, Jordana Rodrigues Barbosa Fradico, Danielle Alves Gomes Zauli, Maria Inês de Miranda Lima, Victor Hugo Melo, Elvis Cristian Cueva Mateo, Luiza de Miranda Lima, Renata Simões |
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| Jazyk: | angličtina |
| Rok vydání: | 2018 |
| Předmět: |
Adult
0301 basic medicine medicine.medical_specialty sexually transmitted diseases polymerase chain reaction 030106 microbiology chlamydia Mycoplasma hominis cervical intraepithelial neoplasia Real-Time Polymerase Chain Reaction medicine.disease_cause Cervical intraepithelial neoplasia urologic and male genital diseases 03 medical and health sciences 0302 clinical medicine medicine Humans 030212 general & internal medicine Asymptomatic Infections Colposcopy Chlamydia biology medicine.diagnostic_test Obstetrics business.industry HPV infection Obstetrics and Gynecology virus diseases Gynecology and obstetrics Uterine Cervical Dysplasia medicine.disease biology.organism_classification female genital diseases and pregnancy complications Cross-Sectional Studies RG1-991 Female Chlamydia trachomatis Mycoplasma genitalium business Ureaplasma urealyticum papillomavirus infections |
| Zdroj: | Revista Brasileira de Ginecologia e Obstetrícia, Vol 40, Iss 9, Pp 540-546 (2018) Revista Brasileira de Ginecologia e Obstetrícia v.40 n.9 2018 Revista brasileira de ginecologia e obstetrícia Federação Brasileira das Sociedades de Ginecologia e Obstetrícia (FEBRASGO) instacron:FEBRASGO |
| ISSN: | 0100-7203 |
| Popis: | Objective To determine the frequency of sexually transmitted infections (STIs) in asymptomatic women and the association of STIs with cervical intraepithelial neoplasia (CIN). Methods A cross-sectional study was performed, enrolling women examined in a general gynecology clinic and in a colposcopy referral center from October 2014 to October 2015. The colposcopy group consisted of 71 women, and the general gynecology group consisted of 55 women. Cervical samples were collected for cervical cytology and a multiplex real-time polymerase chain reaction (PCR) was developed to detect human papillomavirus (HPV) and the STIs caused by the following microorganisms: Chlamydia trachomatis, Mycoplasma hominis, Mycoplasma genitalium, Ureaplasma urealyticum, and Neisseria gonorrhoeae. A multivariate analysis was performed by logistic regression, considering the significance level of 0.05. Results The general frequency of STIs was: 46.8% (HPV); 27.8% (C. trachomatis); 28.6% (M. genitalium); 0.8% (M. hominis); 4.8% (U. urealyticum); and 4.8% (N. gonorrhoeae). The significant risk factors for CIN were: HPV infection (odds ratio [OR] = 2.53; p = 0.024); C. trachomatis (OR = 3.04; p = 0.009); M. genitalium (OR = 2.37; p = 0.04); and HPV and C. trachomatis coinfection (OR = 3.11; p = 0.023). After the multivariate analysis, a significant association was found between HPV and CIN (OR = 2.48; 95% confidence interval [95%CI]: 1.04–5.92; p = 0.04); and between C. trachomatis and CIN (OR = 2.69; 95%CI: 1.11–6.53; p = 0.028). Conclusion The frequency of STIs was high in asymptomatic patients. Infections by HPV and C. trachomatis were independently associated with the presence of CIN. The high frequency of STIs in asymptomatic women suggests the need for routine screening of these infections. |
| Databáze: | OpenAIRE |
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