Suppression of palmitic acid-induced hepatic oxidative injury by neohesperidin-loaded pectin-chitosan decorated nanoliposomes
| Autor: | Mohammad Rezaul Islam Shishir, Wei Chen, Ahmed K. Rashwan, Naymul Karim, Huihui Ke |
|---|---|
| Rok vydání: | 2021 |
| Předmět: |
Palmitic Acid
02 engineering and technology Biochemistry Palmitic acid 03 medical and health sciences chemistry.chemical_compound Structural Biology Humans Viability assay Molecular Biology 030304 developmental biology Neohesperidin Chitosan 0303 health sciences Liposome Hesperidin Biological activity General Medicine Glutathione 021001 nanoscience & nanotechnology Controlled release Oxidative Stress Liver chemistry Liposomes Biophysics Pectins 0210 nano-technology Oxidation-Reduction Intracellular |
| Zdroj: | International Journal of Biological Macromolecules. 183:908-917 |
| ISSN: | 0141-8130 |
| DOI: | 10.1016/j.ijbiomac.2021.05.010 |
| Popis: | The biological activity of neohesperidin (NH, a flavanone glycoside) is limited due to instability in the physiological environment. Thus, the current study aimed to explore the protective effect of NH-loaded pectin-chitosan decorated liposomes (P-CH-NH-NL) against palmitic acid (PA)-induced hepatic oxidative injury in L02 cells. The particles were characterized using DLS, TEM, HPLC, DSC, and cellular uptake study. Then, the protective effect of NH-loaded liposomal systems (NH-NLs) against PA-induced oxidative injury was evaluated in terms of cell viability study, intracellular ROS, superoxide ions (O2-), MMP, and cellular GSH determination. Our results exhibited that NH-NLs significantly lessened the PA-induced hepatic oxidative injury in L02 cells via decreasing ROS and O2- generation, reducing MMP collapse, and attenuating GSH reduction, whereas the free NH samples were ineffective. Furthermore, the coated NH-NLs were more effective than that of uncoated nanoliposome. Overall, our study confirmed that P-CH-NH-NL was capable of reducing PA-induced hepatic oxidative injury. Therefore, the pectin-chitosan decorated nanoliposome can be considered as an efficient delivery system for enhancing cellular uptake of lipophilic compound with controlled release and greater biological activity. |
| Databáze: | OpenAIRE |
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