Viability of Equine Chondrocytes After Exposure to Mepivacaine and Ropivacaine In Vitro
Autor: | Gabriele Biavaschi da Silva, Heloisa Einloft Palma, Kalyne Bertolin, Camila Cantarelli, Alexandre Krause, Alfredo Q. Antoniazzi, Luciana Maria Fontanari Krause, Julien Wergutz, Karin Erica Brass, Flávio Desessards De La Corte, Miguel Gallio |
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Rok vydání: | 2018 |
Předmět: |
040301 veterinary sciences
medicine.medical_treatment Mepivacaine Chondrocyte Flow cytometry 0403 veterinary science Andrology chemistry.chemical_compound Chondrocytes medicine Animals Ropivacaine Propidium iodide Horses Saline Cells Cultured medicine.diagnostic_test Equine 0402 animal and dairy science 04 agricultural and veterinary sciences 040201 dairy & animal science Bupivacaine Staining medicine.anatomical_structure chemistry Trypan blue medicine.drug |
Zdroj: | Journal of equine veterinary science. 77 |
ISSN: | 0737-0806 |
Popis: | Chondrocyte health is altered when exposed to local anesthetics, raising concerns as to the long-term effects of local anesthetics intra-articularly for diagnosis and analgesia. To investigate the drug with the lowest toxic potential, the effect of ropivacaine and mepivacaine on chondrocytes was evaluated. Articular cartilage from normal metacarpophalangeal joints of five equine cadaver specimens was used to establish chondrocyte cultures. Following seven days, chondrocytes were exposed to standard culture medium (DMEM), ropivacaine 7.5 mg/ml (ROP7.5), ropivacaine 10 mg/ml (ROP10), mepivacaine 20 mg/ml (MEP20), mepivacaine 30 mg/ml (MEP 30), and 0.9% saline solution (SAL). Chondrocyte viability was evaluated by trypan blue exclusion, MTT, and flow cytometry via cellular staining with propidium iodide. No differences were observed between treatments following trypan blue exclusion assay. A difference was observed between DMEM and all other treatment groups (P.0001) with a significant viability drop using the MTT assay. Mepivacaine 20 mg/ml and MEP30 exposure between showed greatest decrease in cellular viability compared to SAL, ROP7.5, and ROP10 (P.0001). Cellular viability decreased as measured by flow cytometry in all groups compared to DMEM and ROP7.5 (P.02). Interestingly, the trypan blue, MTT, and flow cytometry assays yielded different results. Although there was no difference using trypan blue, MTT demonstrated that ropivacaine-treated cells had lower viability than DMEM, and cytometry found that ROP7.5 did not differ from DMEM. Results in vitro suggest that short-term exposure to ropivacaine may result in less chondrotoxicity than mepivacaine. In vivo studies are warranted investigating long-term effects of local anesthetics on equine articular cartilage. |
Databáze: | OpenAIRE |
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