The transpeptidase PBP2 governs initial localization and activity of the major cell-wall synthesis machinery in E. coli
| Autor: | Baptiste Cordier, Andrey Aristov, Eva Wollrab, Mariette Matondo, Gizem Özbaykal, Thibault Chaze, Antoine Vigouroux, Sven van Teeffelen, François Simon |
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| Přispěvatelé: | Morphogénèse et Croissance microbiennes / Microbial Morphogenesis and Growth, Institut Pasteur [Paris], Université Paris Diderot, Sorbonne Paris Cité, Paris, France, Université Paris Diderot - Paris 7 (UPD7), Biologie de Synthèse - Synthetic biology, Université Paris-Descartes, Sorbonne Paris Cité, 75014 Paris, France, Spectrométrie de Masse pour la Biologie – Mass Spectrometry for Biology (UTechS MSBio), Institut Pasteur [Paris]-Institut de Chimie du CNRS (INC)-Centre National de la Recherche Scientifique (CNRS), H2020 European Research Council 679980French National Research Agency ANR-10-LABX-62-IBEIDVolkswagenRégion Ile-de-FranceCampus France, ANR-10-LABX-0062,IBEID,Integrative Biology of Emerging Infectious Diseases(2010), European Project: 679980,H2020,ERC-2015-STG,RCSB(2016), Institut Pasteur [Paris] (IP), Institut Pasteur [Paris] (IP)-Institut de Chimie du CNRS (INC)-Centre National de la Recherche Scientifique (CNRS) |
| Jazyk: | angličtina |
| Rok vydání: | 2020 |
| Předmět: |
genetic structures
QH301-705.5 MreB cytoskeleton Science infectious disease bacterial morphogenesis Curvature medicine.disease_cause MreB General Biochemistry Genetics and Molecular Biology Cell wall synthesis Cell wall 03 medical and health sciences chemistry.chemical_compound 0302 clinical medicine Cell Wall physics of living systems Escherichia coli medicine Penicillin-Binding Proteins Rod complex [SDV.BBM]Life Sciences [q-bio]/Biochemistry Molecular Biology Biology (General) single-enzyme dynamics 030304 developmental biology Microbiology and Infectious Disease 0303 health sciences General Immunology and Microbiology Escherichia coli Proteins General Neuroscience microbiology E. coli [SDV.BBM.BM]Life Sciences [q-bio]/Biochemistry Molecular Biology/Molecular biology General Medicine chemistry Cytoplasm Direct binding Biophysics Medicine Peptidoglycan sense organs cell-wall insertion 030217 neurology & neurosurgery Subcellular Fractions Research Article |
| Zdroj: | eLife eLife, eLife Sciences Publication, 2020, 9, ⟨10.7554/eLife.50629⟩ eLife, Vol 9 (2020) eLife, 2020, 9, ⟨10.7554/eLife.50629⟩ |
| ISSN: | 2050-084X |
| Popis: | International audience; Bacterial shape is physically determined by the peptidoglycan cell wall. The cell-wall-synthesis machinery responsible for rod shape in Escherichia coli is the processive 'Rod complex'. Previously, cytoplasmic MreB filaments were thought to govern formation and localization of Rod complexes based on local cell-envelope curvature. Using single-particle tracking of the transpeptidase and Rod-complex component PBP2, we found that PBP2 binds to a substrate different from MreB. Depletion and localization experiments of other putative Rod-complex components provide evidence that none of those provide the sole rate-limiting substrate for PBP2 binding. Consistently, we found only weak correlations between MreB and envelope curvature in the cylindrical part of cells. Residual correlations do not require curvature-based Rod-complex initiation but can be attributed to persistent rotational motion. We therefore speculate that the local cell-wall architecture provides the cue for Rod-complex initiation, either through direct binding by PBP2 or through an unknown intermediate. |
| Databáze: | OpenAIRE |
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