RNA sequencing reveals retinal transcriptome changes in STZ-induced diabetic rats
| Autor: | Zhi‑Yun Lian, Hui-Jun Yang, Geng Liu, Hong‑Ying Zhou, Yuan‑Jie Liu |
|---|---|
| Jazyk: | angličtina |
| Rok vydání: | 2016 |
| Předmět: |
0301 basic medicine
Blood Glucose Male Cancer Research differentially expressed genes Sequence analysis Down-Regulation Biology Real-Time Polymerase Chain Reaction Biochemistry Retina Streptozocin Diabetes Mellitus Experimental Transcriptome Rats Sprague-Dawley 03 medical and health sciences Gene expression Genetics medicine RNA-sequence Electroretinography Animals Molecular Biology Gene Sequence Analysis RNA Gene Expression Profiling RNA Reproducibility of Results Articles Streptozotocin Molecular medicine Molecular biology Up-Regulation Gene expression profiling diabetic retinopathy 030104 developmental biology Gene Ontology Oncology Molecular Medicine medicine.drug |
| Zdroj: | Molecular Medicine Reports |
| ISSN: | 1791-3004 1791-2997 |
| Popis: | The present study aimed to investigate changes in retinal gene expression in streptozotocin (STZ)‑induced diabetic rats using next‑generation sequencing, utilize transcriptome signatures to investigate the molecular mechanisms of diabetic retinopathy (DR), and identify novel strategies for the treatment of DR. Diabetes was chemically induced in 10‑week‑old male Sprague‑Dawley rats using STZ. Flash‑electroretinography (F‑ERG) was performed to evaluate the visual function of the rats. The retinas of the rats were removed to perform high throughput RNA sequence (RNA‑seq) analysis. The a‑wave, b‑wave, oscillatory potential 1 (OP1), OP2 and ∑OP amplitudes were significantly reduced in the diabetic group, compared with those of the control group (P |
| Databáze: | OpenAIRE |
| Externí odkaz: |
|