FGF receptors control vitamin D and phosphate homeostasis by mediating renal FGF-23 signaling and regulating FGF-23 expression in bone
| Autor: | Noemie Beluch, Christelle Stamm, Swann Gaulis, Simon Wöhrle, Marcel Scheibler, Diana Graus-Porta, Francesco Hofmann, Olivier Bonny, Bernd Kinzel, William R. Sellers, Nancy E. Hynes, Joseph Brueggen, Anne Thuery, Matthias Müller |
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| Rok vydání: | 2011 |
| Předmět: |
Fibroblast growth factor 23
medicine.medical_specialty Endocrinology Diabetes and Metabolism FGFR Inhibition Blotting Western Parathyroid hormone Biology urologic and male genital diseases Fibroblast growth factor Kidney Real-Time Polymerase Chain Reaction Bone and Bones Cell Line Mice CYP24A1 Internal medicine medicine Vitamin D and neurology Animals Homeostasis Orthopedics and Sports Medicine Vitamin D Klotho Mice Inbred BALB C Receptors Fibroblast Growth Factor Cell biology Fibroblast Growth Factors Fibroblast Growth Factor-23 Endocrinology Gene Expression Regulation Fibroblast growth factor receptor Signal Transduction |
| Zdroj: | Journal of bone and mineral research : the official journal of the American Society for Bone and Mineral Research. 26(10) |
| ISSN: | 1523-4681 |
| Popis: | The functional interaction between fibroblast growth factor 23 (FGF-23) and Klotho in the control of vitamin D and phosphate homeostasis is manifested by the largely overlapping phenotypes of Fgf23- and Klotho-deficient mouse models. However, to date, targeted inactivation of FGF receptors (FGFRs) has not provided clear evidence for an analogous function of FGFRs in this process. Here, by means of pharmacologic inhibition of FGFRs, we demonstrate their involvement in renal FGF-23/Klotho signaling and elicit their role in the control of phosphate and vitamin D homeostasis. Specifically, FGFR loss of function counteracts renal FGF-23/Klotho signaling, leading to deregulation of Cyp27b1 and Cyp24a1 and the induction of hypervitaminosis D and hyperphosphatemia. In turn, this initiates a feedback response leading to high serum levels of FGF-23. Further, we show that FGFR inhibition blocks Fgf23 transcription in bone and that this is dominant over vitamin D-induced Fgf23 expression, ultimately impinging on systemic FGF-23 protein levels. Additionally, we identify Fgf23 as a specific target gene of FGF signaling in vitro. Thus, in line with Fgf23- and Klotho-deficient mouse models, our study illustrates the essential function of FGFRs in the regulation of vitamin D and phosphate levels. Further, we reveal FGFR signaling as a novel in vivo control mechanism for Fgf23 expression in bone, suggesting a dual function of FGFRs in the FGF-23/Klotho pathway leading to vitamin D and phosphate homeostasis. |
| Databáze: | OpenAIRE |
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