Binary and ternary complexes between T-cell receptor, class II MHC and superantigen in vitro
| Autor: | Jonathan R. Lamb, Alpna Seth, Lawrence J. Stern, Don C. Wiley, Richard D. Klausner, Tom H. M. Ottenhoff, Isaac Engel, Michael John Owen |
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| Rok vydání: | 1994 |
| Předmět: |
Receptors
Antigen T-Cell alpha-beta Bacterial Toxins Molecular Sequence Data chemical and pharmacologic phenomena C-C chemokine receptor type 7 Major histocompatibility complex Immune tolerance Enterotoxins Superantigen Humans Amino Acid Sequence Receptor Multidisciplinary Superantigens biology T-cell receptor HLA-DR1 Antigen hemic and immune systems T lymphocyte biological factors In vitro Cell biology Kinetics Immunology biology.protein Electrophoresis Polyacrylamide Gel |
| Zdroj: | Nature. 369(6478) |
| ISSN: | 0028-0836 |
| Popis: | SUPERANTIGENS are proteins that in association with class II major histocompatibility complex (MHC)-bearing cells can stimulate vir-tually all T cells that express particular classes of the variable β-domains of the T-cell receptor (TCR)1. This mechanism of T-cell activation circumvents the usual requirement for peptide-specific MHC recognition. Staphylococcus aureus enter otoxin B (SEB) is a bacterial superantigen that causes food poisoning and shock2–5. We have characterized the tertiary complex of SEB, a soluble T-cell receptor, and a soluble class II MHC molecule DR1, and the three binary complexes TCR–SEB, SEB–DR1, and the peptide-specific complex DR1–TCR. We report here that in each case the specificity of the interaction among the soluble molecules is the same as observed in biological assays. Native gel electrophoresis and plasmon resonance affinity measurements indicate that SEB–TCR complex can form in the absence of class II MHC and that SEB–TCR interaction increases the binding of DR1. The observation that a superantigen can form complexes with TCR in both the absence and presence of class II MHC may provide a mechanism for its ability to induce anergy in some circumstances and activation in others6,7 (reviewed in ref. 8). |
| Databáze: | OpenAIRE |
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