Identification of a periodontal pathogen and bihormonal cells in pancreatic islets of humans and a mouse model of periodontitis
Autor: | Rosann S Marattil, Terry G. Unterman, Neil M O'Brien-Simpson, Vladimir Ilievski, Klara Valyi-Nagy, Keiko Watanabe, Tibor Valyi-Nagy, Barton Wicksteed, Peter T. Toth, Brian T. Layden, Eric C. Reynolds, Haider W. Aljewari, Stefan J. Green |
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Jazyk: | angličtina |
Rok vydání: | 2020 |
Předmět: |
0301 basic medicine
Male medicine.medical_specialty lcsh:Medicine Pathogenesis Biology Article Periodontal pathogen Prediabetic State 03 medical and health sciences Islets of Langerhans Mice 0302 clinical medicine Insulin resistance Endocrinology Internal medicine Glucose Intolerance medicine Hyperinsulinemia Bacteroidaceae Infections Diabetes Mellitus Animals Humans Prospective Studies Periodontitis lcsh:Science Porphyromonas gingivalis Multidisciplinary Pancreatic islets lcsh:R medicine.disease biology.organism_classification Gingipain Mice Inbred C57BL Disease Models Animal Epidemiologic Studies 030104 developmental biology medicine.anatomical_structure 030220 oncology & carcinogenesis lcsh:Q Insulin Resistance Pancreas |
Zdroj: | Scientific Reports, Vol 10, Iss 1, Pp 1-14 (2020) Scientific Reports |
ISSN: | 2045-2322 |
Popis: | Results from epidemiological and prospective studies indicate a close association between periodontitis and diabetes. However the mechanisms by which periodontal pathogens influence the development of prediabetes/diabetes are not clear. We previously reported that oral administration of a periodontal pathogen, Porphyromonas gingivalis (Pg) to WT mice results in insulin resistance, hyperinsulinemia, and glucose intolerance and that Pg translocates to the pancreas. In the current study, we determined the specific localization of Pg in relation to mouse and human pancreatic α- and β-cells using 3-D confocal and immunofluorescence microscopy and orthogonal analyses. Pg/gingipain is intra- or peri-nuclearly localized primarily in β-cells in experimental mice and also in human post-mortem pancreatic samples. We also identified bihormonal cells in experimental mice as well as human pancreatic samples. A low percentage of bihormonal cells has intracellular Pg in both humans and experimental mice. Our data show that the number of Pg translocated to the pancreas correlates with the number of bihormonal cells in both mice and humans. Our findings suggest that Pg/gingipain translocates to pancreas, particularly β-cells in both humans and mice, and this is strongly associated with emergence of bihormonal cells. |
Databáze: | OpenAIRE |
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