T and B Lymphocyte Transcriptional States Differentiate between Sensitized and Unsensitized Individuals in Alpha-Gal Syndrome

Autor: Shailesh K. Choudhary, Onyinye I. Iweala, Scott P. Commins, Claire T. Addison
Rok vydání: 2021
Předmět:
Male
0301 basic medicine
red meat allergy
Transcription
Genetic
T-Lymphocytes
Lymphocyte
Lymphocyte Activation
lcsh:Chemistry
0302 clinical medicine
Gene expression
alpha-gal syndrome
lcsh:QH301-705.5
Spectroscopy
Aged
80 and over
B-Lymphocytes
biology
General Medicine
Middle Aged
tick
Computer Science Applications
Killer Cells
Natural
medicine.anatomical_structure
Female
IgE
Antibody
Food Hypersensitivity
Adult
Adolescent
alpha-gal
Antigen presentation
Article
Catalysis
Diagnosis
Differential
Inorganic Chemistry
Young Adult
03 medical and health sciences
Immune system
Antigen
medicine
Humans
Physical and Theoretical Chemistry
Molecular Biology
B cell
Aged
Gene Expression Profiling
Organic Chemistry
Computational Biology
Allergens
Immunoglobulin E
Gene expression profiling
Red Meat
030104 developmental biology
lcsh:Biology (General)
lcsh:QD1-999
030228 respiratory system
Case-Control Studies
Immunology
Leukocytes
Mononuclear
biology.protein
Immunization
Zdroj: International Journal of Molecular Sciences
Volume 22
Issue 6
International Journal of Molecular Sciences, Vol 22, Iss 3185, p 3185 (2021)
ISSN: 1422-0067
Popis: The mechanisms of pathogenesis driving alpha-gal syndrome (AGS) are not fully understood. Differences in immune gene expression between AGS individuals and non-allergic controls may illuminate molecular pathways and targets critical for AGS development. We performed immune expression profiling with RNA from the peripheral blood mononuclear cells (PBMCs) of seven controls, 15 AGS participants, and two participants sensitized but not allergic to alpha-gal using the NanoString nCounter PanCancer immune profiling panel, which includes 770 genes from 14 different cell types. The top differentially expressed genes (DEG) between AGS subjects and controls included transcription factors regulating immune gene expression, such as the NFκB pathway (NFKBIA, NFKB2, REL), antigen presentation molecules, type 2/allergic immune responses, itch, and allergic dermatitis. The differential expression of genes linked to T and B cell function was also identified, including transcription factor BCL-6, markers of antigen experience (CD44) and memory (CD27), chemokine receptors (CXCR3, CXCR6), and regulators of B-cell proliferation, cell cycle entry and immunoglobulin production (CD70). The PBMCs from AGS subjects also had increased TNF and IFN-gamma mRNA expression compared to controls. AGS is associated with a distinct gene expression profile in circulating PBMCs. DEGs related to antigen presentation, antigen-experienced T-cells, and type 2 immune responses may promote the development of alpha-gal specific IgE and the maintenance of AGS.
Databáze: OpenAIRE
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