Oestriol and oestradiol increase cell to cell communication and connexin43 protein expression in human myometrium
| Autor: | D.L. Becker, Wei-Li Di, Gillian C. L. Lachelin, H. H. G. Mcgarrigle, N.S.B. Thomas |
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| Rok vydání: | 2001 |
| Předmět: |
Adult
Embryology medicine.medical_specialty medicine.drug_class Blotting Western Uterus Connexin Cell Communication Biology Internal medicine Genetics medicine Humans Molecular Biology Microinjection Cells Cultured Aged Estradiol Estriol Myometrium Gap junction Obstetrics and Gynecology Cell Biology Middle Aged medicine.anatomical_structure Endocrinology Reproductive Medicine Estrogen In utero Connexin 43 Female hormones hormone substitutes and hormone antagonists Developmental Biology |
| Zdroj: | MHR: Basic science of reproductive medicine. 7:671-679 |
| ISSN: | 1460-2407 1360-9947 |
| DOI: | 10.1093/molehr/7.7.671 |
| Popis: | Oestradiol increases the protein expression of connexin43 (Cx43) gap junctions in myometrium but the effect of oestriol on gap junction expression has not been described previously. Oestriol is the most abundant free oestrogen in pregnant women and there is a marked surge in oestriol concentrations before term and idiopathic preterm labour. In order to determine whether oestriol may have a physiological action on the myometrium, cultured human myometrial cells obtained from non-pregnant hysterectomy specimens were exposed to 10 nmol/l oestradiol or oestriol. Intercellular communication between myometrial cells was investigated by microinjection of confluent cultured cells with the gap junction-permeant tracer Cascade Blue. There was a progressive increase in coupling after exposure to oestradiol or oestriol (P < 0.0005). An increase in Cx43 protein expression was demonstrated by immunocytochemistry after 1 h (P < 0.01) and 3 days (P < 0.01) exposure, and by Western blotting after 1 h (P < 0.01) and 3 days (P < 0.05) exposure, to both oestradiol and to oestriol. We conclude that oestriol increases gap junction communication in human myometrium by increasing gap junction expression. Elevated oestriol concentrations may thus play a role in the initiation of labour in women, by increasing cell-cell communication in the myometrium. |
| Databáze: | OpenAIRE |
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