MicroRNA-494 promotes cervical cancer proliferation through the regulation of PTEN
| Autor: | Yan‑E Gao, Yong‑Kang Yang, Wen‑Yan Xi, Jing‑Yuan Li, Ru‑Xing Xi, Qin Li |
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| Rok vydání: | 2014 |
| Předmět: |
Adult
Cancer Research Down-Regulation Uterine Cervical Neoplasms Biology medicine.disease_cause Phosphatidylinositol 3-Kinases Cell Line Tumor microRNA medicine Tensin PTEN Humans RNA Messenger Protein kinase B 3' Untranslated Regions PI3K/AKT/mTOR pathway Cell Proliferation Neoplasm Staging Akt/PKB signaling pathway Reverse Transcriptase Polymerase Chain Reaction Carcinoma PTEN Phosphohydrolase Cancer General Medicine medicine.disease Prognosis Up-Regulation Gene Expression Regulation Neoplastic MicroRNAs Oncology Cancer research biology.protein Female Carcinogenesis Proto-Oncogene Proteins c-akt HeLa Cells Signal Transduction |
| Zdroj: | Oncology reports. 33(5) |
| ISSN: | 1791-2431 |
| Popis: | The phosphoinositide 3-kinase (PI3K)/Akt signaling pathway appears to be a key regulator in cervical carcinogenesis. The phosphatase and tensin homolog deleted on chromosome 10 (PTEN) protein is principally involved in the homeostatic maintenance of PI3K/Akt signaling and PTEN has been identified to play an important role in the occurrence and development of cervical cancer. MicroRNA (miRNA)-494 has been proven to be involved in the carcinogenesis and development of various types of cancer by directly targeting PTEN. However the role, mechanism and clinical significance of miR-494 in cervical cancer have not been further reported. In the present study, we analyzed the expression of miR-494 in -with PTEN expression and clinicopathological data of cervical cancer patients. The results showed that miR-494 expression was significantly upregulated in human cervical cancer cell lines and tissues. miR-494 upregulation was significantly associated with PTEN downregulation, adverse clinicopathological characteristics, poor overall and progression-free survival and poor prognosis. In vitro experiments showed that inhibition of miR-494 suppressed cell proliferation and growth by directly targeting the 3'-untranslated region (3'-UTR) of PTEN mRNA. These findings identified a novel molecular mechanism involved in the regulation of PTEN expression and cervical cancer progression. Results of the present study indicated that miR-494 may have an essential role in the carcinogenesis and progression of cervical cancer and targeting miR-494 may be a promising therapeutic strategy for the treatment of cervical cancer. |
| Databáze: | OpenAIRE |
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