A Water Extract of Pueraria Lobata Inhibited Cytotoxicity of Enterovirus 71 in a Human Foreskin Fibroblast Cell Line
| Autor: | Jung-San Chang, Jih-Jin Tsai, Lien-Chai Chiang, Fu-Min Su, Kuo-Chih Wang |
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| Rok vydání: | 2008 |
| Předmět: |
Male
Pueraria Time Factors Cell Survival Foreskin Pharmacology Cell Line Lobata Cell Adhesion medicine Enterovirus 71 Humans chemoprevention Cytotoxic T cell Cytotoxicity Cytopathic effect Medicine(all) lcsh:R5-920 therapy Pueraria lobata biology Traditional medicine Plant Extracts enterovirus EV71 Water General Medicine Fibroblasts Virus Internalization biology.organism_classification medicine.disease medicine.anatomical_structure lcsh:Medicine (General) Encephalitis |
| Zdroj: | Kaohsiung Journal of Medical Sciences, Vol 24, Iss 10, Pp 523-530 (2008) |
| ISSN: | 2410-8650 1607-551X |
| DOI: | 10.1016/s1607-551x(09)70011-7 |
| Popis: | Enterovirus 71 (EV71) can cause brain encephalitis and mortality. However, effective vaccines or chemotherapeutic agents are not yet available. We tested the hypothesis that Pueraria lobata could inhibit the cytotoxic effect of EV71 in a human foreskin fibroblast cell line by the XTT method. Our results showed that the water extract of P. lobata could inhibit cytopathy induced by EV71 when given before (p < 0.0001), simultaneously with (p < 0.0001), or after viral infection (p < 0.0001). Water extract of P. lobata was effective and its minimal concentration that inhibited 50% of the cytopathic effect (IC50) was 0.028 μg/mL. P. lobata was also safe with a selectivity index greater than 107,000. Water extract of P. lobata appeared to inhibit viral attachment (p < 0.0001) and penetration (p < 0.0001). The anti-EV71 activity of the water extract of P. lobata was not mediated by interferons. In conclusion, the water extract of P. lobata was effective in the management of the disease induced by EV71 infection. |
| Databáze: | OpenAIRE |
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