EFFICIENCY OF BONE MARROW PRECURSOR CELL COLONY-FORMING AS A PREDICTOR OF DISEASE COURSE IN PLASMA CELL MYELOMA PATIENTS WITH A HISTORY OF RADIATION EXPOSURE

Autor: T F Liubarets, Zh. M. Minchenko, O. O. Dmytrenko, V O Moyseyenko, T Yu Shlyakhtichenko, Yu O Silayev, V V Balan, V G Bebeshko
Rok vydání: 2020
Předmět:
0301 basic medicine
Adult
Male
medicine.medical_specialty
Induced Pluripotent Stem Cells
Antineoplastic Agents
Bone Marrow Cells
Stage ii
Gastroenterology
Disease course
Colony-Forming Units Assay
03 medical and health sciences
0302 clinical medicine
Bone Marrow
Precursor cell
Internal medicine
Plasma Cell Myeloma
Antineoplastic Combined Chemotherapy Protocols
Medicine
Humans
Radiology
Nuclear Medicine and imaging
Stage (cooking)
Aged
Neoplasm Staging
business.industry
Macrophages
Stem Cells
Remission Induction
Middle Aged
Radiation Exposure
Granulocyte-Macrophage Colony Forming Unit
Survival Analysis
Radiation exposure
030104 developmental biology
medicine.anatomical_structure
Chernobyl Nuclear Accident
030220 oncology & carcinogenesis
Female
Bone marrow
business
Multiple Myeloma
Granulocytes
Zdroj: Problemy radiatsiinoi medytsyny ta radiobiolohii. 25
ISSN: 2313-4607
Popis: Assessment of role of the bone marrow colony-forming efficiency in plasma cell myeloma patients at different stages of treatment as a prognostic criterion for the disease course.The colony forming efficiency (CFE) was assayed in stage I-II plasma cell myeloma (PCM)patients (n = 37) aged 42-73, namely in patients survived after the Chornobyl NPP accident (n = 21) and persons notexposed to ionizing radiation (n = 16). There were 11 males exposed to ionizing radiation and having got stage I PCM,9 males and 3 females exposed and having got stage II PCM, 3 males and 3 females not exposed and having got stageI PCM, 6 males and 2 females not exposed and having got stage II PCM. Healthy persons (n = 20) were included in thecontrol group.Number of the bone marrow (BM) granulocyte-macrophage colony-forming units (CFU-GM) in both exposedand not exposed PCM patients depended on a disease stage. CFU-GM was (16.7 ± 1.2) in the stage I PCM patients vs.(11.1 ± 1.1) in the stage II PCM ones both being lower (p0.05) compared to control (64.5 ± 2.2). Changes in cluster formation were similar, i.e. (37.7 ± 1.6) and (19.4 ± 1.3) correspondingly in the stage I and stage II PCM patients.Respective values in control were (89.8 ± 3.6). The CFE in stage I and stage II PCM patients at the time of diagnosiswas lower (5.7 ± 1.5 and 2.4 ± 1.1 respectively) vs. control (39.5 ± 1.51, p0.05), but has increased in remission upto (29. 6 ± 1.8) and (13.8 ± 1.2) respectively. There was no difference at that between the irradiated and non-irradiated patients. Number of the fibroblast colony-forming units (CFU-F) in the stage I and stage II PCM patients duringdiagnosis, namely (43.9 ± 5.4) and (22.5 ± 3.7), was lower (p0.05) vs. control (110.5 ± 4.9). Upon reaching remission the CFU-F value increased significantly (p0.05), reaching (87.4 ± 4.2) and (55.6 ± 2.7) correspondingly in thestage I and stage II PCM patients.Dependence of the BM cell CFE on the stage of PCM and presence or absence of remission was established. Prognostic value of the CFE of BM CFU-GM in terms of life span of patients was shown (Ro Spearm = 0.39,p0.02), namely in case of CFE20 before the polychemotherapy administration the life span of PCM patients wassignificantly longer vs. cases of CFE20.Meta roboty: na osnovi doslidzhennia efektyvnosti koloniieutvorennia klityn kistkovogo mozku khvorykh na plazmoklitynnu miielomu na riznykh etapakh likuvannia otsinyty ïï znachennia iak prognostychnogo kryteriiu perebigu zakhvoriuvannia.Materialy i metody. Efektyvnist' koloniieutvorennia (EKU) otsineno u 37 patsiientiv vikom 42–73 rokiv z plazmoklitynnoiu miielomoiu (PKM) I–II ct.: 21 patsiienta, postrazhdalogo vnaslidok avariï na Chornobyl's'kiĭ AES, ta16 neoprominenykh khvorykh. Sered oprominenykh z I st. obstezheno 11 cholovikiv, sered khvorykh z II st. – 9 cholovikivta 3 zhinok, sered neoprominenykh z I st. – 3 cholovikiv, 3 zhinok, sered patsiientiv z II st. – 6 cholovikiv ta 2 zhinok.Kontrol'nu grupa sklaly 20 zdorovykh osib.Rezul'taty. Kil'kist' koloniieutvoriuiuchykh granulotsytarno-makrofagal'nykh odynyts' (KUO-GM) kistkovogo mozku (KM) khvorykh na PKM ob’iednanoï grupy (opromineni, neopromineni) zalezhala vid stadiï zakhvoriuvannia. U khvorykh z I st. KUO-GM stanovyla 16,7 ± 1,2 proty 11,1 ± 1,1 pry II st. i bula nyzhchoiu (p0,05), porivniano z kontrolem (64,5 ± 2,2). Podibnymy buly zminy pry utvorenni klasteriv (I st. – 37,7 ± 1,6; II st. – 19,4 ± 1,3; kontrol' –89,8 ± 3,6). U khvorykh z I ta II st. na moment diagnostyky EKU bula nyzhchoiu (5,7 ± 1,5 ta 2,4 ± 1,1 vidpovidno),porivniano z kontrolem (39,5 ± 1,51, p0,05), pry remisiï EKU zrostala (29,6 ± 1,8 ta 13,8 ± 1,2 vidpovidno). Pokaznyky oprominenykh i neoprominenykh patsiientiv ne vidriznialys'. Na etapi diagnostyky kil'kist' koloniieutvoriuiuchykh odynyts' fibroblastiv (KUO-F) bula nyzhchoiu (p0,05), porivniano z kontrolem (110,5 ± 4,9) – 43,9 ± 5,4 ta22,5 ± 3,7 u patsiientiv z I ta II st. PKM vidpovidno. Pry dosiagnenni remisiï KUO-F zrostala (p0,05): u khvorykh zI st. PKM KUO-F siagala 87,4 ± 4,2; u patsiientiv z II st. – 55,6 ± 2,7.Vysnovky. Vstanovlena zalezhnist' EKU klityn KM vid stadiï PKM ta naiavnosti/vidsutnosti remisiï. Pokazanoprognostychne znachennia pokaznyka EKU KUO-GM KM shchodo tryvalosti zhyttia patsiientiv (Ro Spearm = 0,39, p0,02):za naiavnosti EKU20 do provedennia polikhimioterapiï tryvalist' zhyttia khvorykh na PKM bula suttievo dovshoiuporivniano z patsiientamy, u iakykh pokaznyk EKU20.
Databáze: OpenAIRE
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