Effect of Ischemia–Reperfusion on Na+,K+-ATPase Expression in Human Liver Tissue Allograft: Image Analysis by Confocal Laser Scanning Microscopy
| Autor: | Yves Patrice Le Treut, Benkoel L, Anne-Marie Benoliel, Dominique Lombardo, Dodero F, Chamlian A, Jean Hardwigsen, Eric Mas, Danielle Botta-Fridlund |
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| Rok vydání: | 2004 |
| Předmět: |
Adult
Male Physiology ATPase Microfilament Sensitivity and Specificity law.invention Cohort Studies Tissue Culture Techniques Cell membrane Ischemia Reference Values Confocal microscopy law Cadaver medicine Humans Transplantation Homologous Na+/K+-ATPase Probability Analysis of Variance Microscopy Confocal Tight junction biology Biopsy Needle Gastroenterology Middle Aged Immunohistochemistry Tissue Donors Liver Transplantation Transplantation medicine.anatomical_structure Membrane Liver Biochemistry Reperfusion Injury Hepatocytes biology.protein Biophysics Female Sodium-Potassium-Exchanging ATPase Biomarkers |
| Zdroj: | Digestive Diseases and Sciences. 49:1387-1393 |
| ISSN: | 0163-2116 |
| DOI: | 10.1023/b:ddas.0000042235.72622.16 |
| Popis: | We have analyzed the effect of ischemia-reperfusion on expression of hepatic Na+,K+-ATPase on bile canalicular (BCM) and basolateral membranes (BLM) in human liver allografts using confocal laser scanning microscopy imaging. Na+, K+-ATPase, an integral membrane enzyme, plays a key role in the physiology and structure of hepatocytes, where it maintains the electrochemical gradients for Na+ and K+ across the cell membrane. The concentrations of these ions as well as their gradients regulate the active transport across the plasma membrane for bile acid and water from sinusoidal to canalicular membranes. In addition, Na+,K+-ATPase is also involved in cellular structure because of its close relationship with submembrane microfilaments and its implication in tight junction assembly. Therefore, Na+,K+-ATPase appears as an indicator of tissue viability and hepatic functionality during liver transplantation. Its localization and its function in BCM are still controversial. As in previous studies, we found an enzyme expression in both BLM and BCM. We show that ischemia induced a decrease in Na+,K+-ATPase expression only in BCM. This result could be explained by the differences in biochemical membrane environment between basolateral and bile canalicular Na+,K+-ATPase. Membrane lipid fluidity, which is more elevated in BLM than in BCM, could protect the enzyme during ischemia. After reperfusion, Na+,K+-ATPase expression was strongly decreased in both BCM and BLM. This alteration following reperfusion is probably due to multiple factors: direct alteration of the enzyme catalytic subunit and modification of its environment and membrane lipid fluidity by free radicals and changes in ATP levels and ionic distribution. This important decrease in Na+,K+-ATPase expression of both BLM and BCM could disturb not only hepatic secretory function but also cellular volume and structure during the postoperative period. |
| Databáze: | OpenAIRE |
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