Isolation of myosin messenger ribonucleoprotein particles which contain a protein fraction affecting myosin synthesis
| Autor: | Doris S. Kennedy, Durrheim Ga, AndréJ Bester, Stuart M. Heywood |
|---|---|
| Rok vydání: | 1980 |
| Předmět: |
Biophysics
Fraction (chemistry) Chick Embryo macromolecular substances Myosins Biology Biochemistry Myosin Tobacco mosaic virus Animals RNA Messenger Molecular Biology Triticum Messenger RNA Muscles fungi RNA A protein Translation (biology) Cell Biology Plants Messenger RNP Nucleoproteins Ribonucleoproteins Protein Biosynthesis Ribosomes |
| Zdroj: | Biochemical and Biophysical Research Communications. 92:524-531 |
| ISSN: | 0006-291X |
| DOI: | 10.1016/0006-291x(80)90365-4 |
| Popis: | Myosin mRNP particles were purified using metrizamide buoyant density gradient centrifugation. This mRNP particle contains only the 26S myosin mRNA and, therefore, is a purified mRNP with regard to mRNA composition. A protein fraction, eluting from DEAE-cellulose between 0.15M KC l and 0.28M KC l , was iso=lated from the purified myosin mRNP particle. A significant stimulation of myosin heavy chain synthesis is observed when the isolated fraction is added to an unfractionated wheat germ cell-free system containing myosin mRNA. This protein fraction was found to have no effect on the translation of TMV RNA. In addition, this protein fraction does not bind TMV RNA, but is associated with myosin mRNA during translation. |
| Databáze: | OpenAIRE |
| Externí odkaz: |
|