Tonsillitis exacerbates renal injury in IgA nephropathy through promoting Th22 cells chemotaxis
| Autor: | Fengmin Shao, Chen Chen, Huiming Luo, Xiaozhao Li, Mengyuan Zhu, Lu Gan, Qiaoling Zhou, Jiaxi Pu, Ting Meng |
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| Rok vydání: | 2017 |
| Předmět: |
0301 basic medicine
Nephrology Adult Male medicine.medical_specialty Chemokine Urology Tonsillitis 030232 urology & nephrology Enzyme-Linked Immunosorbent Assay urologic and male genital diseases Statistics Nonparametric Nephropathy Pathogenesis 03 medical and health sciences 0302 clinical medicine Th2 Cells stomatognathic system Internal medicine Medicine Humans Cells Cultured Chemokine CCL22 Cell chemotaxis Analysis of Variance biology business.industry Chemotaxis Biopsy Needle Glomerulonephritis IGA respiratory system Acute Kidney Injury medicine.disease Flow Cytometry Immunohistochemistry Coculture Techniques 030104 developmental biology medicine.anatomical_structure Tonsil Case-Control Studies Immunology biology.protein Disease Progression CCL27 Female business |
| Zdroj: | International urology and nephrology. 50(7) |
| ISSN: | 1573-2584 |
| Popis: | Tonsillitis can promote the progression of IgA nephropathy (IgAN) by aggravating immunopathologic response. Th22 cell disorder is involved in the pathogenesis of IgAN with tonsillitis. This study was determined to explore the possible mechanism of IgAN with tonsillitis underlying Th22 cell chemotaxis response to the effect of CCL20, CCL22, and CCL27. This research was conducted on 65 subjects including 16 healthy controls (HC group), 5 patients with renal carcinoma (HTC group) and 44 patients with IgAN between 2015 and 2016. According to clinical symptoms and results of throat swab culture, patients with IgAN were divided into two groups: IgAN with tonsillitis (IgAN + tonsillitis, n = 14) and IgAN patients without tonsillitis (IgAN, n = 30). Distribution of Th22 cells in IgAN patients was determined. The expression of CCL20, CCL22, and CCL27 in both peripheral blood and kidneys of IgAN patients was investigated. Severity of pathological lesions in IgAN patients was analyzed. Coculture assay and transwell assay were performed to explore the impacts of human mesangial cells (HMC) on Th22 cell chemotaxis and Th22 cell local accumulation under hemolytic streptococcus (HS) infection. Th22 cell percentages in IgAN patients increased compared with healthy controls. This increased Th22 cell percentage was positively correlated with the renal lesions of IgAN patients. Correspondingly, the expression of CCL20, CCL22, and CCL27 in renal tissue increased in IgAN patients. Tonsillitis exacerbated these overrepresentations of Th22 cells and chemokines. It was found that HMC could produce CCL20, CCL22, and CCL27. The supernatant of HMC was chemotactic for Th22 cells. This activity of HMC was stimulated by HS infection, whereas treatment of anti-CCL20, anti-CCL22, and anti-CCL27 antibodies partly blocked this chemoattractant effect of HMC. Tonsil infection may aggravate the renal pathological lesions of IgAN by exacerbating Th22 cell accumulation. Our data suggested a collaboration between HMC and Th22 cells in IgAN with tonsillitis underlying the effects of CCL20, CCL22, and CCL27. |
| Databáze: | OpenAIRE |
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