Cystic proliferation of germline stem cells is necessary to reproductive success and normal mating behavior in medaka
| Autor: | Luisa F Arias Padilla, Rafael Henrique Nóbrega, Diana C Castañeda-Cortés, Ricardo Shohei Hattori, Juan I. Fernandino, Omar David Moreno Acosta, Ivana F. Rosa |
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| Přispěvatelé: | INTECH (CONICET-UNSAM), Universidade Estadual Paulista (Unesp), Sao Paulo Fisheries Institute (APTA/SAA) |
| Rok vydání: | 2020 |
| Předmět: |
0301 basic medicine
Male mating vigor Oryzias Cell Cycle Proteins Germline purl.org/becyt/ford/1 [https] Sexual Behavior Animal 0302 clinical medicine Transforming Growth Factor beta Mating Biology (General) In Situ Hybridization General Neuroscience Intracellular Signaling Peptides and Proteins General Medicine Stem Cells and Regenerative Medicine Cell biology medicine.anatomical_structure Gamete Medicine Female Stem cell Research Article QH301-705.5 Science ndrg1b Biology General Biochemistry Genetics and Molecular Biology gametogenesis reproduction 03 medical and health sciences Meiosis ndrg1 medicine Animals purl.org/becyt/ford/1.6 [https] CRISPR/Cas9 Gametogenesis Cell Proliferation mitosis General Immunology and Microbiology Reproductive success 030104 developmental biology Germ Cells Other Developmental biology 030217 neurology & neurosurgery Developmental Biology |
| Zdroj: | eLife eLife, Vol 10 (2021) Scopus Repositório Institucional da UNESP Universidade Estadual Paulista (UNESP) instacron:UNESP CONICET Digital (CONICET) Consejo Nacional de Investigaciones Científicas y Técnicas instacron:CONICET |
| ISSN: | 2050-084X |
| Popis: | The production of an adequate number of gametes is necessary for normal reproduction, for which the regulation of proliferation from early gonadal development to adulthood is key in both sexes. Cystic proliferation of germline stem cells is an especially important step prior to the beginning of meiosis; however, the molecular regulators of this proliferation remain elusive in vertebrates. Here, we report that ndrg1b is an important regulator of cystic proliferation in medaka. We generated mutants of ndrg1b that led to a disruption of cystic proliferation of germ cells. This loss of cystic proliferation was observed from embryogenic to adult stages, impacting the success of gamete production and reproductive parameters such as spawning and fertilization. Interestingly, the depletion of cystic proliferation also impacted male sexual behavior, with a decrease of mating vigor. These data illustrate why it is also necessary to consider gamete production capacity in order to analyze reproductive behavior. Fil: Arias Padilla, Luisa Fernanda. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - La Plata. Instituto de Investigaciones Biotecnológicas. Instituto de Investigaciones Biotecnológicas "Dr. Raúl Alfonsín" (sede Chascomús). Universidad Nacional de San Martín. Instituto de Investigaciones Biotecnológicas. Instituto de Investigaciones Biotecnológicas "Dr. Raúl Alfonsín" (sede Chascomús); Argentina Fil: Castañeda Cortés, Diana C.. Universidade Estadual Paulista Julio de Mesquita Filho; Brasil Fil: Rosa, Ivana F. Universidade Estadual Paulista Julio de Mesquita Filho; Brasil Fil: Moreno Acosta, Omar David. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - La Plata. Instituto de Investigaciones Biotecnológicas. Instituto de Investigaciones Biotecnológicas "Dr. Raúl Alfonsín" (sede Chascomús). Universidad Nacional de San Martín. Instituto de Investigaciones Biotecnológicas. Instituto de Investigaciones Biotecnológicas "Dr. Raúl Alfonsín" (sede Chascomús); Argentina Fil: Hattori, Ricardo S.. Universidade Estadual Paulista Julio de Mesquita Filho; Brasil Fil: Nóbrega, Rafael H.. Universidade Estadual Paulista Julio de Mesquita Filho; Brasil Fil: Fernandino, Juan Ignacio. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - La Plata. Instituto de Investigaciones Biotecnológicas. Instituto de Investigaciones Biotecnológicas "Dr. Raúl Alfonsín" (sede Chascomús). Universidad Nacional de San Martín. Instituto de Investigaciones Biotecnológicas. Instituto de Investigaciones Biotecnológicas "Dr. Raúl Alfonsín" (sede Chascomús); Argentina |
| Databáze: | OpenAIRE |
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