Correlation betweenEnterococcus faecalisBiofilms Development Stage and Quantitative Surface Roughness Using Atomic Force Microscopy
| Autor: | Francisco Vassiliepe Sousa Arruda, Cibele Barreto Mano de Carvalho, Victor Alves Carneiro, Gustavo Arruda Bezerra, Benildo Sousa Cavada, Alexandre Havt, Lara de Queiroz Viana Braga, Ricardo Pires dos Santos, Edson Holanda Teixeira, Valder N. Freire, Taiana Maia de Oliveira, Theodora T.P. Arruda |
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| Rok vydání: | 2008 |
| Předmět: |
Materials science
biology Surface Properties Micropore Filters Biofilm Analytical chemistry Collodion Surface finish biochemical phenomena metabolism and nutrition Microscopy Atomic Force biology.organism_classification Enterococcus faecalis Culture Media Biofouling chemistry.chemical_compound Extracellular polymeric substance chemistry Biofilms Surface roughness Biophysics Brain heart infusion Humans Cellulose Instrumentation |
| Zdroj: | Microscopy and Microanalysis. 14:150-158 |
| ISSN: | 1435-8115 1431-9276 |
| DOI: | 10.1017/s1431927608080227 |
| Popis: | Biofilms are assemblages of microorganisms and their associated extracellular products at an interface and typically with an abiotic or biotic surface. The study of the morphology of biofilms is important because they are associated with processes of biofouling, corrosion, catalysis, pollutant transformation, dental caries, drug resistance, and so forth. In the literature, biofilms have been examined by atomic force microscopy (AFM), which has proven to be a potent tool to study different aspects of the biofilm development on solid surfaces. In this work, we used AFM to investigate topographical changes during the development process ofEnterococcus faecalisbiofilms, which were generated on sterile cellulose nitrate membrane (CNM) filters in brain heart infusion (BHI) broth agar blood plates after 24, 36, 72, 192, and 360 h. AFM height images showed topographical changes due to biofilm development, which were used to characterize several aspects of the bacterial surface, such as the presence of extracellular polymeric substance, and the biofilm development stage. Changes in the development stage of the biofilm were shown to correlate with changes in the surface roughness as quantified through the mean roughness. |
| Databáze: | OpenAIRE |
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