A synthetic peptide derived from domain III envelope glycoprotein of Dengue virus induces neutralizing antibody
| Autor: | Siriporn Chattanadee, R. Shenbagarathai, Akanitt Jittmittraphap, Pornsawan Leaungwutiwong, J. Asnet Mary |
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| Rok vydání: | 2017 |
| Předmět: |
0301 basic medicine
Dengue Vaccines Enzyme-Linked Immunosorbent Assay Cross Reactions Dengue virus Antibodies Viral medicine.disease_cause Dengue fever 03 medical and health sciences Viral Envelope Proteins Neutralization Tests Virology Genetics medicine Animals Neutralizing antibody Molecular Biology Dengue vaccine 030102 biochemistry & molecular biology biology General Medicine Dengue Virus biochemical phenomena metabolism and nutrition biology.organism_classification medicine.disease Antibodies Neutralizing Recombinant Proteins Flavivirus 030104 developmental biology Polyclonal antibodies biology.protein Rabbits Antibody Keyhole limpet hemocyanin |
| Zdroj: | Virus Genes. 54:25-32 |
| ISSN: | 1572-994X 0920-8569 |
| Popis: | Dengue virus (DENV) is an arthropod-borne human pathogen that represents a severe public health threat in both endemic and non-endemic regions. So far, there is no licensed vaccine or specific drugs available for dengue fever. A fifteen-amino-acid-long peptide that includes the NGR motif was chemically synthesized and conjugated with keyhole limpet hemocyanin. A standard immunization protocol was followed for the production of polyclonal antibodies by immunizing rabbits against the synthetic peptide. The immune response elicited high-titer polyclonal antibodies with the reactivity of the anti-peptide antibody against both synthetic peptide and four serotypes of DENV confirmed by DOT-ELISA. Neutralizing activity of anti-peptide antibody was found to be cross-reactive and effective resulting in 60% reduction of infectivity at 1:200 dilution in all four serotypes of DENV. Our findings have the potential to further improve our understanding of virus-host interactions and provide new insights into neutralizing antibodies and could also be used as a drug target. |
| Databáze: | OpenAIRE |
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