Effects of Lipoic Acid Supplementation on Activities of Cyclooxygenases and Levels of Prostaglandins E2 and F2α Metabolites, in the Offspring of Rats with Streptozotocin-Induced Diabetes

Autor: Othman A. Al Hanbali, Ghaleb A. Oriquat, Mahmoud Abu-Samak, Maher D. Salim, Hisham Y. Al-Matubsi
Jazyk: angličtina
Rok vydání: 2016
Předmět:
0301 basic medicine
Blood Glucose
medicine.medical_specialty
Article Subject
Offspring
Endocrinology
Diabetes and Metabolism
Placenta
Pregnancy in Diabetics
Prostaglandin
030209 endocrinology & metabolism
Biology
Dinoprost
lcsh:Diseases of the endocrine glands. Clinical endocrinology
Dinoprostone
Diabetes Mellitus
Experimental
03 medical and health sciences
chemistry.chemical_compound
0302 clinical medicine
Endocrinology
Pregnancy
Internal medicine
Diabetes mellitus
medicine
Animals
Fetus
lcsh:RC648-665
Thioctic Acid
Body Weight
Streptozotocin
medicine.disease
Glutathione
Rats
Lipoic acid
Disease Models
Animal
030104 developmental biology
medicine.anatomical_structure
chemistry
Liver
Prostaglandin-Endoperoxide Synthases
Gestation
Pregnancy
Animal
Female
Corrigendum
medicine.drug
Research Article
Zdroj: Journal of Diabetes Research, Vol 2016 (2016)
Journal of Diabetes Research
ISSN: 2314-6753
2314-6745
Popis: Background.Our aim was to evaluate the protective effect of lipoic acid (LA) on fetal outcome of diabetic mothers.Methods.Diabetes was induced in female rats using streptozotocin and rats were made pregnant. Pregnant control (group 1;n=9; and group 2;n=7) or pregnant diabetic (group 3;n=10; and group 4;n=8) rats were treated daily with either LA (groups 2 and 4) or vehicle (groups 1 and 3) between gestational days 0 and 15. On day 15 of gestation, the fetuses, placentas, and membranes were dissected, examined morphologically, and then homogenized, to measure cyclooxygenase (COX) activities and metabolisms of prostaglandin (PG) E2(PGEM) and PGF2α(PGFM) levels. The level of total glutathione was measured in the maternal liver and plasma and in all fetuses.Results.Supplementation of diabetic rats with LA was found to significantly (p<0.05) reduce resorption rates in diabetic rats and led to a significant (p<0.05) increase in liver, plasma, and fetuses total glutathione from LA-TD rats as compared to those from V-TD. Decreased levels of PGEM and elevated levels of PGFM in the fetuses, placentas, and membranes were characteristic of experimental diabetic gestation associated with malformation. The levels of PGEM in malformed fetuses from LA-TD mothers was significantly (p<0.05) higher than those in malformed fetuses from V-TD rats.Conclusions.LA treatment did not completely prevent the occurrence of malformations. Thus, other factors may be involved in the pathogenesis of the diabetes-induced congenital malformations.
Databáze: OpenAIRE
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