Use of a cell recycle reactor to increase production of a proteolysis-susceptible peptide secreted from recombinant Saccharomyces cerevisiae
| Autor: | Russell Arthur Brierley, Robert Steven Siegel |
|---|---|
| Rok vydání: | 1990 |
| Předmět: |
Proteolysis
Cell Saccharomyces cerevisiae Biomedical Engineering Bioengineering Peptide Biology Growth Hormone-Releasing Hormone Applied Microbiology and Biotechnology Models Biological law.invention law Bioreactor medicine Humans Food science Cloning Molecular chemistry.chemical_classification medicine.diagnostic_test Cell Cycle equipment and supplies biology.organism_classification Recombinant Proteins medicine.anatomical_structure Biodegradation Environmental chemistry Biochemistry Fermentation Recombinant DNA Molecular Medicine Degradation (geology) Mathematics Biotechnology |
| Zdroj: | Bio/technology (Nature Publishing Company). 8(7) |
| ISSN: | 0733-222X |
| Popis: | Operation of a continuous microbial fermentor with cell recycle can significantly reduce degradation-associated loss of a secreted protein product. Under continuous fermentation conditions, proteolysis of a recombinant growth hormone releasing factor (GRF) analog secreted by S. cerevisiae was first order with respect to GRF concentration. The maximal GRF concentration was increased from 5 mg/l to 30 mg/l by the use of a cell recycle reactor, and volumetric productivity was increased more than 10-fold to an average of 10 mg/l-1/h-1. A mathematical model shows that increased productivity in the cell recycle reactor results from a reduced degradation rate and a shorter residence time of the product in the fermentor. |
| Databáze: | OpenAIRE |
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