An imbalance of arachidonic acid metabolism in asthma
| Autor: | Helen G. Morris, Samuel S. Yen |
|---|---|
| Rok vydání: | 1981 |
| Předmět: |
Blood Platelets
medicine.medical_specialty Lipoxygenase Biophysics Arachidonic Acids Biochemistry High-performance liquid chromatography chemistry.chemical_compound Reference Values Internal medicine medicine Humans Platelet Molecular Biology Chromatography High Pressure Liquid Asthma Arachidonic Acid biology Cell Biology Metabolism medicine.disease Arachidonic acid metabolism Endocrinology chemistry Prostaglandin-Endoperoxide Synthases biology.protein Arachidonic acid Cyclooxygenase |
| Zdroj: | Biochemical and biophysical research communications. 103(2) |
| ISSN: | 0006-291X |
| Popis: | Metabolism of arachidonic acid via the cyclooxygenase and lipoxygenase pathways was studied in washed platelets from normal and asthmatic subjects. The platelets were incubated with [1- 14 C] arachidonic acid and the metabolites formed were separated by high pressure liquid chromatography (HPLC). The platelets from asthmatic patients had a 40% decrease in cyclooxygenase-derived metabolites and a 70% increase in lipoxygenase-derived product when compared with metabolites generated by platelets from normal subjects. The ratio of cyclooxygenase to lipoxygenase products was 3.24 ± 0.26 for platelets from normal subjects, and 1.14 ± 0.15 with platelets from the asthmatic patients. These results indicate an imbalance of arachidonic acid metabolism in platelets from asthmatic patients. |
| Databáze: | OpenAIRE |
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