Determination of montelukast sodium in human plasma by column-switching high-performance liquid chromatography with fluorescence detection
| Autor: | Terukazu Takano, Hisao Ochiai, Ken-ichi Hara, Toshio Kamei, Naotaka Uchiyama |
|---|---|
| Rok vydání: | 1998 |
| Předmět: |
Cyclopropanes
Male Calibration curve Ultraviolet Rays Analytical chemistry Administration Oral Acetates Sulfides High-performance liquid chromatography Sensitivity and Specificity Fluorescence spectroscopy medicine Humans Anti-Asthmatic Agents Montelukast Chromatography High Pressure Liquid Chromatography Chemistry Elution Stereoisomerism General Chemistry Fluorescence Spectrometry Fluorescence Montelukast Sodium Quinolines Leukotriene Antagonists Quantitative analysis (chemistry) medicine.drug |
| Zdroj: | Journal of chromatography. B, Biomedical sciences and applications. 713(2) |
| ISSN: | 1387-2273 |
| Popis: | MK-0476 (montelukast sodium) is a potent and selective cysteinyl leukotriene receptor antagonist that is being investigated in the treatment of asthma. A simple and sensitive method for the determination of MK-0476 in human plasma was developed using column-switching high-performance liquid chromatography (HPLC) with fluorescence detection. A plasma sample was injected directly onto the HPLC system consisting of a pre-column (Capcell pak MF) and an analytical column (Capcell pak C18) which were connected with a six-port switching valve. The column eluate was monitored with a fluorescence detector (excitation at 350 nm; emission at 400 nm). The calibration curve was linear in a concentration range of 1-500 ng ml(-1) for MK-0476 in human plasma. The intra-day coefficients of variation of all concentrations within the range was less than 9.2%, and the intra-day accuracy values were between 97.2 and 114.6%. This method was used to measure the plasma concentration of MK-0476 following oral administration of the drug in humans. |
| Databáze: | OpenAIRE |
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