Mouse lymphoma thymidine kinase gene mutation assay: follow-up International Workshop on Genotoxicity Test Procedures, New Orleans, Louisiana, April 2000
| Autor: | Hiroyasu Shimada, Kathryn Flanders, Colin Riach, Stephen McEnaney, Julie Clements, Stephan Kirchner, Leon F. Stankowski, Robert Preston, George Bolcsfoldi, Jo Oliver, Takumi Awogi, Marie-Claude Ouldelhkim, Richard H.C. San, Joann Kraycer, Michael D. Fellows, B. Bhaskar Gollapudi, Peter Jenkinson, Paul Kirby, Kamala Pant, Wolfgang Muster, Maria A. Cifone, Deborah D. Collard, Masamitsu Honma, Michael R. O’Donovan, Martha M. Moore, Brian Myhr, Karen Harrington-Brock |
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| Rok vydání: | 2002 |
| Předmět: |
medicine.medical_specialty
Time Factors Lymphoma Epidemiology Health Toxicology and Mutagenesis education Guidelines as Topic Data entry medicine.disease_cause Thymidine Kinase Education Toxicology Mice Soft agar medicine Animals Medical physics Workgroup Genetics (clinical) Test procedures Thymidine Kinase Gene business.industry Mutagenicity Tests Mouse Lymphoma Microsoft excel Mutation business Genotoxicity |
| Zdroj: | Environmental and molecular mutagenesis. 40(4) |
| ISSN: | 0893-6692 |
| Popis: | The Mouse Lymphoma Assay (MLA) Workgroup of the International Workshop on Genotoxicity Test Procedures held a second harmonization meeting just prior to the U.S. Environmental Mutagen Society Meeting in New Orleans, LA, in April 2000. The discussion focused on several important aspects of the MLA, including: 1) cytotoxicity measures and their determination, 2) use of a 24-hr treatment, 3) the ability of the assay to detect aneugens, and 4) concentration selection. Prior to the meeting the group developed Microsoft Excel Workbooks for data entry. Ten laboratories entered their data into the workbooks (primarily as coded chemicals). The Excel Workbooks were used to facilitate data analysis by generating an extensive set of graphs that were evaluated by the meeting participants. Based on the Workgroup's previous agreement that a single cytotoxicity measure should be established for both the microwell and soft agar versions of the assay, the Workgroup analyzed the submitted data and unanimously agreed that the relative total growth (RTG) should be used as the cytotoxicity measure for concentration selection and data evaluation. The Workgroup also agreed that the various cytotoxicity measures should be calculated using the same methods regardless of whether the soft agar or microwell version of the assay was used. In the absence of sufficient data to make a definitive determination, the Workgroup continued to endorse the International Committee on Harmonization recommendation for the use of 24-hr treatment and made some specific 24-hr treatment protocol recommendations. The Workgroup recognized the ability of the MLA to detect at least some aneugens and also developed general guidance and requirements for appropriate concentration selection. |
| Databáze: | OpenAIRE |
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