Evolutionary analysis of the APA genes in the Phaseolus genus: wild and cultivated beans species as sources of lectin-related resistance factors?
| Autor: | Incoronata Galasso, Lucia Lioi, Francesca Sparvoli, Roberto Bollini, C. Lanave, Maria Gloria Daminati |
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| Jazyk: | angličtina |
| Rok vydání: | 2007 |
| Předmět: |
R Factors
Locus (genetics) Evolution Molecular Molecular evolution Gene duplication Genetics Gene family Humans Phytohemagglutinins Gene Southern blot Phaseolus APA locus common bean resistance factors Phylogenetic tree biology General Medicine biology.organism_classification Multigene Family lectin Plant Lectins alpha-Amylases Agronomy and Crop Science Biotechnology |
| Zdroj: | Theoretical and applied genetics 115 (2007): 959–970. doi:10.1007/s00122-007-0622-1 info:cnr-pdr/source/autori:Lioi L.; Galasso I.; Lanave C.; Daminati M.G.; Bollini R.; F. Sparvoli./titolo:Evolutionary analysis of the APA genes in the Phaseolus genus: wild and cultivated beans species as sources of lectin-related resistance factors?/doi:10.1007%2Fs00122-007-0622-1/rivista:Theoretical and applied genetics/anno:2007/pagina_da:959/pagina_a:970/intervallo_pagine:959–970/volume:115 |
| DOI: | 10.1007/s00122-007-0622-1 |
| Popis: | The APA (Arcelin/Phytohemagglutinin/alpha-Amylase inhibitor) gene family is composed of various members, present in Phaseolus species and coding for lectin and lectin-related seed proteins having the double role of storage and defense proteins. Here members of the APA family have been identified by immunological, functional, and molecular analyses and representative genes were sequenced in nine wild species of Phaseolus. All taxa possessed at least one member of the true lectin gene. No arcelin type sequences have been isolated from the species examined. Among the wild species studied, only P. costaricensis contained an alpha-amylase inhibitor (alpha-AI). In addition P. augusti, P. maculatus, P. microcarpus, and P. oligospermus showed the presence of the lectin-related alpha-amylase inhibitor-like (AIL) genes and alpha-AI activity. Data from Southern blot analysis indicated the presence of only one lectin gene in P. parvulus and P. filiformis, while an extensive gene duplication of the APA locus was found in the other Phaseolus species. Phylogenetic analysis carried out on the nucleotide sequences showed the existence of two main clusters and clearly indicated that lectin-related genes originated from a paralogous duplication event preceding the development of the ancestor to the Phaseolus genus. The finding of detectable alpha-AI activity in species containing AIL genes suggests that exploiting APA genes variability in the Phaseolus genus may represent a valuable tool to find new members that may have acquired insecticidal activities. |
| Databáze: | OpenAIRE |
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