Evaluation of Disk carbapenemase test using improved disks for rapid detection and differentiation of clinical isolates of carbapenemase-producing Enterobacterales
| Autor: | Yunsop Chong, Jung Lim Kim, Myungsook Kim, Hyukmin Lee, Kyungwon Lee, Younghee Seo, Da Hee Jung, Dongeun Yong |
|---|---|
| Rok vydání: | 2020 |
| Předmět: |
0301 basic medicine
Microbiology (medical) Imipenem 030106 microbiology Modified method Biology Rapid detection beta-Lactamases Microbiology 03 medical and health sciences 0302 clinical medicine Bacterial Proteins Enterobacterales Republic of Korea polycyclic compounds medicine Humans Pharmacology (medical) 030212 general & internal medicine Enterobacteriaceae Infections Carbapenemase producing biochemical phenomena metabolism and nutrition Tertiary care hospital bacterial infections and mycoses body regions Infectious Diseases medicine.drug |
| Zdroj: | Journal of infection and chemotherapy : official journal of the Japan Society of Chemotherapy. 27(8) |
| ISSN: | 1437-7780 |
| Popis: | Objectives Rapid detection of carbapenemase-producing Enterobacterales (CPE) is important to control spread of the resistance. We previously reported that imipenem disks prepared from injectable imipenem-cilastatin could rapidly detect KPC- and NDM-type carbapenemases. In the present study, we evaluated performance of disks of IPM and combined disks of imipenem-tazobactam and imipenem-EDTA, which were prepared from powders of imipenem and inhibitors. Methods Isolates of Enterobacterales were recovered from specimens of patients at a tertiary care hospital in Korea during January 2017 and March 2018. Routine CPE detection was performed by the CPE surveillance personnel whereas evaluation of the Disk carbapenemase test (DCT) was performed by the other personnel without knowing the results of surveillance. The DCT was carried out by pressing disks on to colonies and rehydrating in Petri plates and observing color change. Results The DCT differentiated 688 of 694 (sensitivity 99.1%) carbapenemase-producing isolates in 2.5–20 min: 630 with KPC, 51 with NDM, three with IMP, one with VIM, two with KPC and IMP, and one with NDM and OXA-181. The DCT failed to detect six OXA- 48-like enzyme-producing isolates, but the modified method using 96-well flat-bottom microplates with mineral oil cover detected all 29 OXA-48-like enzyme-producing isolates in 20–120 min. The DCT was negative for all 440 ertapenem-nonsusceptible, carbapenemase gene-negative isolates (specificity 100%). Conclusion The procedure of DCT is simple and can differentiate isolates of Enterobacterales with KPC-, NDM-, IMP- and VIM-type carbapenemases rapidly, and the modified DCT can detect isolates with OXA-48-like enzymes rapidly. |
| Databáze: | OpenAIRE |
| Externí odkaz: |
|
Full Text from ScienceDirect