Genomic evolution of antimicrobial resistance in Escherichia coli
| Autor: | Leekitcharoenphon, Pimlapas, Johansson, Markus Hans Kristofer, Munk, Patrick, Malorny, Burkhard, Skarzynska, Magdalena, Wadepohl, Katharina, Moyano, Gabriel, Hesp, Ayla, Veldman, Kees T., Bossers, Alex, Graveland, Haitske, van Essen, Alieda, Battisti, Antonio, Caprioli, Andrea, Blaha, Thomas, Hald, Tine, Daskalov, Hristo, Saatkamp, Helmut W., Staerk, Katharina D. C., Luiken, Roosmarijn E. C., Van Gompel, Liese, Hansen, Rasmus Borup, Dewulf, Jeroen, Duarte, Ana Sofia Ribeiro, Zajac, Magdalena, Wasyl, Dariusz, Sanders, Pascal, Gonzalez-Zorn, Bruno, Brouwer, Michael S. M., Wagenaar, Jaap A., Heederik, Dick J. J., Mevius, Dik, Aarestrup, Frank M., EFFORT Consortium, for the |
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| Přispěvatelé: | Technical University of Denmark [Lyngby] (DTU), Bundesinstitut für Risikobewertung - Federal Institute for Risk Assessment (BfR), Universidad Complutense de Madrid = Complutense University of Madrid [Madrid] (UCM), Wageningen BioVeterinary Research, Wageningen University and Research [Wageningen] (WUR), Laboratoire de Fougères - ANSES, Agence nationale de sécurité sanitaire de l'alimentation, de l'environnement et du travail (ANSES), European Project: 613754,EC:FP7:KBBE,FP7-KBBE-2013-7-single-stage,EFFORT(2013), Danmarks Tekniske Universitet = Technical University of Denmark (DTU), IRAS OH Epidemiology Microbial Agents, Klinische infectiologie en microb. lab., dI&I I&I-4, dIRAS RA-I&I RA, Faculteit Diergeneeskunde |
| Jazyk: | angličtina |
| Rok vydání: | 2021 |
| Předmět: |
0301 basic medicine
Genomic alteration antibiotic resistance Swine Epidemiology résistance aux antibiotiques veterinary drug medicine.disease_cause Genome Poultry Feces animal évolution bacteria Escherichia coli Infections Phylogeny médicament humainélevage 2. Zero hunger Genetics human drug bactérie Multidisciplinary Virulence Bacteriologie transmission Genomics Bacteriology Host Pathogen Interaction & Diagnostics Text Anti-Bacterial Agents Europe Medicine médicament médicament vétérinaire Bioinformatica & Diermodellen Science 030106 microbiology Microbial Sensitivity Tests GENETIC-STRUCTURE Biology Article Evolution Molecular 03 medical and health sciences Antibiotic resistance résistance aux antimicrobiens Drug Resistance Bacterial Bio-informatics & Animal models medicine Escherichia coli Animals Life Science Epidemiology Bio-informatics & Animal models ddc:610 Veterinary Sciences antimicrobial resistance microbiologie General genome Host Pathogen Interaction & Diagnostics Epidemiologie Whole genome sequencing génome microbiology Bacteriology [SDV.MP.BAC]Life Sciences [q-bio]/Microbiology and Parasitology/Bacteriology Host Pathogen Interactie & Diagnostiek livestock Red Meat 030104 developmental biology Metagenomics Genetic marker Epidemiologie Bioinformatica & Diermodellen Bacteriologie Host Pathogen Interactie & Diagnostiek Molecular evolution Cattle POULTRY Mobile genetic elements humain Genome Bacterial |
| Zdroj: | Scientific Reports, Vol 11, Iss 1, Pp 1-12 (2021) Scientific Reports Scientific Reports, Nature Publishing Group, 2021, 11 (1), pp.15108. ⟨10.1038/s41598-021-93970-7⟩ SCIENTIFIC REPORTS Scientific Reports, 2021, 11 (1), pp.15108. ⟨10.1038/s41598-021-93970-7⟩ Scientific Reports 11 (2021) Scientific Reports, 11(1). NLM (Medline) Scientific Reports, 11 Leekitcharoenphon, P, Johansson, M H K, Munk, P, Malorny, B, Skarżyńska, M, Wadepohl, K, Moyano, G, Hesp, A, Veldman, K T, Bossers, A, Graveland, H, van Essen, A, Battisti, A, Caprioli, A, Blaha, T, Hald, T, Daskalov, H, Saatkamp, H W, Stärk, K D C, Luiken, R E C, Van Gompel, L, Hansen, R B, Dewulf, J, Duarte, A S R, Zając, M, Wasyl, D, Sanders, P, Gonzalez-Zorn, B, Brouwer, M S M, Wagenaar, J A, Heederik, D J J, Mevius, D, Aarestrup, F M & consortium, EFFORT 2021, ' Genomic evolution of antimicrobial resistance in Escherichia coli ', Scientific Reports, vol. 11, 15108 . https://doi.org/10.1038/s41598-021-93970-7 |
| ISSN: | 2045-2322 |
| DOI: | 10.1038/s41598-021-93970-7 |
| Popis: | The emergence of antimicrobial resistance (AMR) is one of the biggest health threats globally. In addition, the use of antimicrobial drugs in humans and livestock is considered an important driver of antimicrobial resistance. The commensal microbiota, and especially the intestinal microbiota, has been shown to have an important role in the emergence of AMR. Mobile genetic elements (MGEs) also play a central role in facilitating the acquisition and spread of AMR genes. We isolated Escherichia coli (n = 627) from fecal samples in respectively 25 poultry, 28 swine, and 15 veal calf herds from 6 European countries to investigate the phylogeny of E. coli at country, animal host and farm levels. Furthermore, we examine the evolution of AMR in E. coli genomes including an association with virulence genes, plasmids and MGEs. We compared the abundance metrics retrieved from metagenomic sequencing and whole genome sequenced of E. coli isolates from the same fecal samples and farms. The E. coli isolates in this study indicated no clonality or clustering based on country of origin and genetic markers; AMR, and MGEs. Nonetheless, mobile genetic elements play a role in the acquisition of AMR and virulence genes. Additionally, an abundance of AMR was agreeable between metagenomic and whole genome sequencing analysis for several AMR classes in poultry fecal samples suggesting that metagenomics could be used as an indicator for surveillance of AMR in E. coli isolates and vice versa. |
| Databáze: | OpenAIRE |
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