Magneto immunofluorescence assay for diagnosis of celiac disease
| Autor: | S. Fabiano, Silvia R. Hernández, Luis Beltramino, María Isabel Pividori, Nidia Garnero, Marta Wagener, Silvina V. Kergaravat, Liliana Trotta |
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| Rok vydání: | 2013 |
| Předmět: |
Tissue transglutaminase
Enzyme-Linked Immunosorbent Assay Immunofluorescence Biochemistry Antibodies Analytical Chemistry Magnetics GTP-Binding Proteins medicine Humans Environmental Chemistry Protein Glutamine gamma Glutamyltransferase 2 Fluorescent Antibody Technique Indirect Spectroscopy EC50 Transglutaminases biology medicine.diagnostic_test Chemistry Antibody titer Enzymes Immobilized Molecular biology Celiac Disease Titer ROC Curve Anti-transglutaminase antibodies biology.protein Antibody Peroxidase |
| Zdroj: | Analytica Chimica Acta. 798:89-96 |
| ISSN: | 0003-2670 |
| DOI: | 10.1016/j.aca.2013.09.009 |
| Popis: | A magneto immunofluorescence assay for the detection of anti-transglutaminase antibodies (ATG2) in celiac disease was developed. The ATG2 were recognized by transglutaminase enzyme immobilized on the magnetic beads and then the immunological reaction was revealed by antibodies labeled with peroxidase. The fluorescent response of the enzymatic reaction with o-phenylenediamine and H2O2 as substrates was correlated with anti-transglutaminase titer, showing EC50 and LOD values of 1:11,600 and 1:74,500 of antibody titers, respectively. A total number of 29 sera samples from clinically confirmed cases of celiac disease and 19 negative control samples were tested by the novel magneto immunofluorescence assay. The data were submitted to the receiver-operating characteristic plot (ROC) analysis which indicated that 8.1 U was the most effective cut-off value to discriminate correctly between celiac and non-celiac patients. The immunofluorescence assay exhibited a sensitivity of 96.6%, a specificity of 89.5% and an efficiency 93.8% compared with the commercial optical ELISA kit. |
| Databáze: | OpenAIRE |
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