Immunogenotyping with antigen receptor gene probes as a diagnostic tool in childhood acute lymphoblastic leukaemia

Autor: Roger Weil, Andreas Hirt, Martin F. Fey, Edward W. Khandjian, Andreas Tobler, Barbara Stadelmann, Delaleu B, Annette Ridolfi-Lüthy, Wagner Hp, Lore Theilkäs
Rok vydání: 2009
Předmět:
Adolescent
Genotype
Receptors
Antigen
T-Cell
Site-Specific Type II Deoxyribonucleases
T-Lymphocyte gene rearrangement
Deoxyribonuclease HindIII
Biology
Gene Rearrangement
T-Lymphocyte
Deoxyribonuclease EcoRI
Immunophenotyping
Bacterial Proteins
ddc:570
Acute lymphocytic leukemia
T-Cell antigen receptors/genetics
medicine
Humans
Leukemia-Lymphoma
Adult T-Cell
Immunoglobulin Heavy Chains/genetics
Gene Rearrangement
beta-Chain T-Cell Antigen Receptor
Child
Deoxyribonucleases
Type II Site-Specific
Gene
Burkitt Lymphoma/diagnosis/genetics
Southern blot
Gene Rearrangement
T-cell receptor
Infant
Nucleic Acid Hybridization
Adult T-Cell Leukemia-Lymphoma/diagnosis/genetics
Hematology
General Medicine
Gene rearrangement
medicine.disease
Burkitt Lymphoma
Molecular biology
Preschool child
Child
Preschool
Beta-Chain T-Cell Antigen Receptor Gene Rearrangement
Immunology
Immunoglobulin heavy chain
DNA Probes
Immunoglobulin Heavy Chains
Clone (B-cell biology)
Restriction Fragment Length Polymorphism
Polymorphism
Restriction Fragment Length
Zdroj: European Journal of Haematology, Vol. 45, No 4 (1990) pp. 215-222
ISSN: 1600-0609
0902-4441
Popis: 13 cases of childhood acute lymphoblastic leukaemia (ALL) were studied combining cell surface marker analysis with immunogenotyping by Southern blot hybridisation with a panel of antigen receptor gene probes. The immunophenotypes were unequivocal: 7 patients had B-phenotype and 6 patients T-phenotype ALL. In several patients immunogenotypes were not fully consistent with the respective phenotypes. For example, 2 B-cell precursor ALL had rearranged TCR beta chain genes and 2 T-ALL rearrangement of Ig heavy-chain genes. All cases showed clonal rearrangement or deletions within the TCR delta gene locus. TCR delta gene rearrangements might, therefore, serve as markers of clonality but not of B- or T-lineage in immature lymphoid neoplasms. We conclude that in current diagnostic practice immunogenotyping is a supplement rather than an alternative to immunophenotyping by surface marker analysis.
Databáze: OpenAIRE
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